Nguyen Khan Viet, Nguyen Linh Khanh Thi, Le Nhan Trong, Ho Duc Viet, Heinämäki Jyrki, Raal Ain, Nguyen Hoai Thi
Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Ngo Quyen 6, 49000 Hue City, Vietnam.
Institute of Pharmacy, Faculty of Medicine, University of Tartu, Nooruse 1, 50411 Tartu, Estonia.
ACS Omega. 2025 Aug 27;10(35):40313-40323. doi: 10.1021/acsomega.5c05568. eCollection 2025 Sep 9.
Tetrahydropalmatine (THP) is the major bioactive alkaloid in Lour. (Menispermaceae) with well-known pharmacological properties, such as antiaddiction, antitumor, and neuroprotective activities. In this study, a novel ultrasonic-assisted extraction method for the sustainable extraction of the tetrahydropalmatine alkaloid from Lour. tubers was developed and optimized. The study reports also the development and validation of an HPLC-PDA analytical method intended for the quantification of THP in medicinal plant accompanied by a rapid and straightforward sample preparation procedure. A set of environmentally friendly and human-safe solvents was systematically evaluated for their extraction yield of THP, and the response surface methodology (RSM) was used for the optimization of extraction. Among the solvents studied, lactic acid showed the highest extraction yield under the optimized conditions of extraction: 90% lactic acid concentration, solvent-to-material ratio of 40 mL/g, and ultrasonic-assisted extraction at 65 °C for 10 min. With the ultrasound-enhanced extraction, the yield of THP was 1.2-1.4 times higher than that obtained with conventional solvent extraction methods. Moreover, XAD-8 resin demonstrated superior recovery performance with a THP recovery rate of 92.02 ± 0.60%. This is the first study encompassing a "green" ultrasonic-assisted solvent extraction, a validated quantification method, and resin adsorption techniques for the sustainable and efficient extraction and recovery of THP from .
延胡索乙素（THP）是延胡索（防己科）中的主要生物活性生物碱，具有抗成瘾、抗肿瘤和神经保护等众所周知的药理特性。在本研究中，开发并优化了一种从延胡索块茎中可持续提取延胡索乙素生物碱的新型超声辅助提取方法。该研究还报告了一种用于定量药用植物中THP的HPLC-PDA分析方法的开发和验证，以及一种快速简便的样品制备程序。系统评估了一组环境友好且对人体安全的溶剂对THP的提取率，并采用响应面法（RSM）对提取进行优化。在所研究的溶剂中，在优化的提取条件下，乳酸的提取率最高：乳酸浓度为90%，料液比为40 mL/g，在65℃下超声辅助提取10分钟。通过超声强化提取，THP的产量比传统溶剂提取方法高出1.2 - 1.4倍。此外，XAD - 8树脂表现出优异的回收性能，THP回收率为92.02±0.60%。这是第一项涵盖“绿色”超声辅助溶剂提取、经过验证的定量方法以及树脂吸附技术，用于从延胡索中可持续高效提取和回收THP的研究。
