Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan.
Colloids Surf B Biointerfaces. 2013 Oct 1;110:163-70. doi: 10.1016/j.colsurfb.2013.04.037. Epub 2013 Apr 30.
Plants are exploited as a potential source for the large-scale production of noble gold nanoparticles in the recent years owing to their various potential applications in nanobiotechnology and nanomedicine. The present work describes green biosynthetic procedures for the production of gold nanoparticles for the first time by using an aqueous extract of the Dysosma pleiantha rhizome. The biosynthesized gold nanoparticles were confirmed and characterized by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy equipped with energy dispersive spectroscopy. The results revealed that aqueous extract of D. pleiantha rhizome has potential to reduce chloroauric ions into gold nanoparticles and the synthesized gold nanoparticles were showed spherical in shape with an average of 127nm. Further, we investigated the anti-metastatic activity of biosynthesized gold nanoparticles against human fibrosarcoma cancer cell line HT-1080. The results showed that the biosynthesized gold nanoparticles were non-toxic to cell proliferation and, also it can inhibit the chemo-attractant cell migration of human fibrosarcoma cancer cell line HT-1080 by interfering the actin polymerization pathway. Thus, the usage of gold nanoparticles biosynthesized from D. pleiantha rhizome can be used as a potential candidate in the drug and gene delivery to metastatic cancer.
近年来，由于植物在纳米生物技术和纳米医学中的各种潜在应用，它们被用作大规模生产贵金属纳米粒子的潜在来源。本工作首次描述了通过使用重楼根茎的水提物来生产金纳米粒子的绿色生物合成方法。通过紫外可见光谱、傅里叶变换红外光谱、透射电子显微镜和扫描电子显微镜以及能量色散光谱对生物合成的金纳米粒子进行了确认和表征。结果表明，重楼根茎的水提物具有将氯金酸离子还原为金纳米粒子的潜力，合成的金纳米粒子呈球形，平均粒径为 127nm。此外，我们研究了生物合成的金纳米粒子对人纤维肉瘤癌细胞系 HT-1080 的抗转移活性。结果表明，生物合成的金纳米粒子对细胞增殖没有毒性，并且可以通过干扰肌动蛋白聚合途径抑制人纤维肉瘤癌细胞系 HT-1080 的趋化细胞迁移。因此，重楼根茎生物合成的金纳米粒子可作为转移性癌症药物和基因递送的潜在候选物。
