抗原特异性免疫球蛋白 E 的存在不足以引起小鼠过敏性鼻嗜酸性粒细胞浸润。
Existence of antigen-specific immunoglobulin E is not sufficient for allergic nasal eosinophil infiltration in mice.
机构信息
Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
出版信息
Int Arch Allergy Immunol. 2013;161 Suppl 2:125-8. doi: 10.1159/000350565. Epub 2013 May 29.
BACKGROUND
Immunoglobulin E (IgE) is important for the development of allergic rhinitis (AR), though the contribution of IgE to the infiltration of eosinophils in the nasal mucosa has not been fully elucidated. In this study, antigen-induced sneezing and nasal eosinophil accumulation were comparatively investigated in anti-ovalbumin (OVA)-IgE transgenic (Tg) and wild-type (WT) mice.
METHODS
Tg and OVA-immunized WT mice were intranasally challenged with OVA. Antigen-specific serum IgE level, sneezing and infiltration of eosinophil into the nasal cavity were then examined.
RESULTS
The level of serum OVA-specific IgE in Tg mice was significantly higher than that in antigen-immunized WT mice. Compared to saline challenge, intranasal challenge with OVA significantly induced sneezing in both Tg and immunized WT mice. However, antigen-induced nasal eosinophil infiltration was observed in immunized WT mice but not in Tg mice.
CONCLUSIONS
IgE-mediated responses might not play a crucial role in antigen-induced eosinophil infiltration in AR.
背景
免疫球蛋白 E（IgE）对于过敏性鼻炎（AR）的发展很重要，尽管 IgE 对鼻黏膜嗜酸性粒细胞浸润的贡献尚未完全阐明。在这项研究中，我们比较研究了抗卵清蛋白（OVA）-IgE 转基因（Tg）和野生型（WT）小鼠中的抗原诱导性打喷嚏和鼻内嗜酸性粒细胞积聚。
方法
用 OVA 对 Tg 和 OVA 免疫的 WT 小鼠进行鼻腔内挑战。然后检查了针对抗原的血清 IgE 水平、打喷嚏和嗜酸性粒细胞浸润到鼻腔的情况。
结果
Tg 小鼠的血清 OVA 特异性 IgE 水平明显高于抗原免疫的 WT 小鼠。与盐水挑战相比，OVA 鼻内挑战在 Tg 和免疫 WT 小鼠中均显著诱导打喷嚏。然而，在免疫 WT 小鼠中观察到抗原诱导的鼻内嗜酸性粒细胞浸润，但在 Tg 小鼠中未观察到。
结论
IgE 介导的反应在 AR 中的抗原诱导性嗜酸性粒细胞浸润中可能不起关键作用。
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