Electrophoresis. 2013 Sep;34(18):2725-32. doi: 10.1002/elps.201300088.
The bioconjugation of quantum dots (QDs) is a key process in their application for bioanalysis as well as imaging. The coupling of QDs with biologically active molecules such as peptides, nucleic acids, and/or antibodies enables their fluorescent labeling, and therefore, selective and sensitive tracking during the bioanalytical process, however, the efficiency of the labeling and preservation of the biological activity of the bioconjugate have to be considered. In this study, a new approach of the bioconjugation of CdTe-QDs and human immunoglobulin employing a small peptide is described. The heptapeptide (HWRGWVC) was synthesized and characterized by mass spectrometry, liquid chromatography, and capillary electrophoresis. Moreover, the peptide was used as a capping agent for QDs synthesis. The CdTe-QDs were synthesized by microwave synthesis (600 W, 20 min) using 3.2 mM CdCl2 and 0.8 mM Na2TeO3. The bioconjugation of QDs capped by this peptide with immunoglobulin was investigated by capillary electrophoresis and magnetic immunoextraction coupled with electrochemical detection by differential pulse voltammetry. Furthermore, the applicability of prepared bioconjugates for fluorescent immunodetection was verified using immobilized goat antihuman IgG antibody.
量子点（QD）的生物偶联是将其应用于生物分析和成像的关键过程。将 QD 与生物活性分子（如肽、核酸和/或抗体）偶联，可实现其荧光标记，从而在生物分析过程中进行选择性和灵敏性跟踪，然而，标记的效率和生物缀合物的生物活性的保持都必须加以考虑。在本研究中，描述了一种利用小肽进行 CdTe-QD 和人免疫球蛋白的生物偶联的新方法。七肽（HWRGWVC）通过质谱、液相色谱和毛细管电泳进行了合成和表征。此外，该肽被用作 QD 合成的封端剂。CdTe-QD 通过微波合成（600 W，20 min），使用 3.2 mM CdCl2 和 0.8 mM Na2TeO3 进行合成。通过毛细管电泳和磁免疫萃取结合差分脉冲伏安法电化学检测，研究了由该肽封端的 QD 与免疫球蛋白的生物偶联。此外，还使用固定化山羊抗人 IgG 抗体验证了制备的生物缀合物用于荧光免疫检测的适用性。
