Zhang Xiao-Ling, Zhang Ling-Ju, Zhu Yu-Qiu, Wang Lin-Hua
Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Provincial Strategic Allance for Innovation in Industry of Dendrobium officinale, Zhejiang Agricultural and Forestry University, Lin'an 311300, China.
Zhongguo Zhong Yao Za Zhi. 2013 Feb;38(4):494-7.
The explants were obtained from stem segments of hybrid combination of Dendrobium officinale germplasms (Zhejiang Yandang Mountain x Yunnan Guangnan). The screened culture mediums for buds induction and multiplication, which were 1/2MS + IBA 1.0 mg x L(-1) +6-BA 1.0 mg x L(-1) and 1/2MS + IBA 1.5 mg x L(-1) +6-BA 0.5 mg x L(-1) respectively, were applied to 159 germplasms of D. officinale from Zhejiang, Yunnan, Guangxi, Hunan, etc. The medium for axillary buds induction had universality with 94.3% induction rate. During buds multiplication, there were significant differences in proliferation effect among germplasms, and three proliferation forms i.e. single bud, multiple buds and protocorm were differentiated from different germplasms. The results showed that different germplasms of D. officinale had specific requirements for culture medium. Therefore, developing special culture medium for breeding clones of D. officinale is urgent and has important application values.
外植体取自铁皮石斛种质（浙江雁荡山×云南广南）杂交组合的茎段。筛选出的用于芽诱导和增殖的培养基，分别为1/2MS + IBA 1.0 mg·L⁻¹ + 6 - BA 1.0 mg·L⁻¹和1/2MS + IBA 1.5 mg·L⁻¹ + 6 - BA 0.5 mg·L⁻¹，将其应用于来自浙江、云南、广西、湖南等地的159份铁皮石斛种质。腋芽诱导培养基具有通用性，诱导率为94.3%。在芽增殖过程中，不同种质间的增殖效果存在显著差异，不同种质分化出单芽、多芽和原球茎三种增殖形式。结果表明，不同铁皮石斛种质对培养基有特定要求。因此，开发针对铁皮石斛克隆育种的专用培养基迫在眉睫且具有重要应用价值。
