[朱砂根的组织培养与植株再生]

[Tissue culture and plant regeneration of Ardisia crenata].

作者信息

Ma Mingdong, Liu Junli, Pu Shangrao

机构信息

School of Forest and Horticulture, Sichuan University of Agriculture, Ya'an 625014, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Aug;34(16):2043-6.

PMID:19938541

Abstract

OBJECTIVE

Our research studied the fast-breeding technology of Ardisia crenata sims by using tissue culture and provided the scientific foundation for industry production.

METHOD

The effects of axillary buds and plant regeneration of different basic medium, hormones and additives on induction and multiplication were studied.

RESULT

The best culture medium for the induction of axillary buds, which took the stems of A. crenate were as explants, was MS + 6-BA 0.5 mg x L(-1) + NAA 0.1 mg x L(-1), and the best medium for multiplication was MS + 6-BA 2.0 mg x L(-1) + NAA 0.1 mg x L(-1) + KT 0.5 mg x L(-1), the best medium for roots generation was 1/2MS + IBA 0.2 mg x L(-1). We also found that the roots'generation, roots rate and mean number of roots can be promoted by adding 0.2% Ac, and the most suitable ground substance was river sand-perlite-vermiculite (1:1:1) or perlite-vermiculite (1:1). With axillary buds and plant regeneration methode, more than 80% A. crenata sims could be regenerated integratedly.

CONCLUSION

A. crenata sims can be regenerated integratedly and breeded fast by using axillary bud proliferation technology.

摘要

目的

本研究采用组织培养技术对朱砂根快繁技术进行研究，为其工业化生产提供科学依据。

方法

研究不同基本培养基、激素及添加剂对朱砂根腋芽诱导和增殖及植株再生的影响。

结果

以朱砂根茎段为外植体诱导腋芽的最佳培养基为MS + 6-BA 0.5 mg·L⁻¹ + NAA 0.1 mg·L⁻¹，增殖最佳培养基为MS + 6-BA 2.0 mg·L⁻¹ + NAA 0.1 mg·L⁻¹ + KT 0.5 mg·L⁻¹，生根最佳培养基为1/2MS + IBA 0.2 mg·L⁻¹。添加0.2%活性炭可促进生根、提高生根率和平均生根数，最适宜的基质为河沙-珍珠岩-蛭石（1:1:1）或珍珠岩-蛭石（1:1）。采用腋芽增殖和植株再生方法，朱砂根的再生完整率可达80%以上。