高果糖玉米糖浆对变形链球菌产酸、黏附和生物膜形成的影响。
Effect of high-fructose corn syrup on the acidogenicity, adherence and biofilm formation of Streptococcus mutans.
机构信息
Department of Endodontics and Operative Dentistry, School of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
出版信息
Aust Dent J. 2013 Jun;58(2):213-8. doi: 10.1111/adj.12074.
BACKGROUND
Although high-fructose corn syrup (HFCS) as a kind of sugar has been widely used in manufactured foods recently, there is little information available regarding its cariogenicity. The aim of this study was to evaluate the cariogenic potential of HFCS.
METHODS
Streptococcus mutans UA159 was inoculated into HFCS media and cultivated. The pH of each culture was measured to assess acidogenicity. Spectrophotometric turbidity was measured to determine the percentage of adherence. Confocal laser scanning microscopy and SYTO-9 staining were employed to observe biofilm formation. Sucrose media was used as a positive control.
RESULTS
The ΔpH in HFCS media was significantly larger than that in sucrose media and the pH in HFCS media decreased faster (p < 0.05). The percentage of adherence of S. mutans in HFCS media was significantly lower than that in sucrose media (p < 0.05). The biofilm formed in sucrose media was significantly thicker than that in HFCS media (p < 0.05).
CONCLUSIONS
The results of this study suggest that the cariogenicity of S. mutans in the presence of HFCS may differ compared to its cariogenicity in the presence of sucrose. Further in vivo studies need to be undertaken to resolve this uncertainty.
背景
尽管高果糖玉米糖浆（HFCS）作为一种糖已广泛用于加工食品，但关于其致龋性的信息却很少。本研究旨在评估 HFCS 的致龋潜能。
方法
将变异链球菌 UA159 接种到 HFCS 培养基中进行培养。测量每个培养物的 pH 值以评估产酸能力。用分光光度比浊法测定黏附率。采用共聚焦激光扫描显微镜和 SYTO-9 染色观察生物膜形成。蔗糖培养基用作阳性对照。
结果
HFCS 培养基中的 ΔpH 值明显大于蔗糖培养基中的 pH 值，且 HFCS 培养基中的 pH 值下降更快（p<0.05）。HFCS 培养基中变异链球菌的黏附率明显低于蔗糖培养基（p<0.05）。蔗糖培养基中形成的生物膜明显比 HFCS 培养基中的厚（p<0.05）。
结论
本研究结果表明，HFCS 存在下变异链球菌的致龋性可能与蔗糖存在下的致龋性不同。需要进行进一步的体内研究来解决这一不确定性。
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