Tanasilovic Srdjan, Medenica Ljiljana, Popadic Svetlana
Department of Dermatovenereology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Clinic of Dermatovenereology, Clinical Center of Serbia, Belgrade, Serbia.
Australas J Dermatol. 2014 Nov;55(4):e74-6. doi: 10.1111/ajd.12067. Epub 2013 May 29.
Direct immunofluorescence of peri-lesional skin is the gold standard in the diagnosis of pemphigus. A specific immunofluorescence pattern may also be demonstrated in the outer root sheath of anagen and telogen hair. We demonstrated an intercellular reticular deposition of immunoglobulin G in the outer root sheath of plucked anagen and telogen hair in all pemphigus vulgaris patients with active disease and for the first time in all patients with active pemphigus foliaceus. Moreover, we demonstrated for the first time that plucked hair samples may be kept at -20°C for at least 2 weeks before immunofluorescent staining and analysis.
皮损周围皮肤的直接免疫荧光检查是天疱疮诊断的金标准。在生长期和休止期毛发的外根鞘中也可显示出特定的免疫荧光模式。我们在所有患有活动性疾病的寻常型天疱疮患者以及所有患有活动性落叶型天疱疮的患者中，首次证实了拔除的生长期和休止期毛发的外根鞘中有免疫球蛋白G的细胞间网状沉积。此外，我们首次证明，拔除的毛发样本在进行免疫荧光染色和分析之前，可以在-20°C下保存至少2周。
