Laboratory of Neurosciences and Behavior, Federal University of Ceará, Rua Cel. Nunes de Melo, 1127, Fortaleza 60430270, Brazil.
Pharmacol Biochem Behav. 2013 Sep;110:1-7. doi: 10.1016/j.pbb.2013.05.012. Epub 2013 May 25.
This study was designed to investigate the beneficial effect of catechin in a model of Parkinson's disease. Unilateral, intrastriatal 6-hydroxydopamine (6-OHDA)-lesioned rats were pretreated with catechin (10 and 30 mg/kg) by intraperitoneal (i.p.) injection 2h before surgery and for 14 days afterwards. After treatments, apomorphine-induced rotations, locomotor activity, working memory and early and late aversive memories were evaluated. The mesencephalon was used to determine the levels of monoamines and measurement of glutathione (GSH). Immunohistochemical staining was also used to evaluate the expression of tyrosine hydroxylase (TH) in mesencephalic and striatal tissues. Catechin administration attenuated the increase in rotational behavior and the decrease in locomotor activity observed in lesioned rats. Although catechin did not rescue the impairment of late aversive memory, it protected the animals against 6-OHDA-induced working memory deficits. Furthermore, catechin treatment restored GSH levels, and significantly increased dopamine and DOPAC content, and TH-immunoreactivity in 6-OHDA-lesioned rats. Catechin protected 6-OHDA-lesioned rats due to its antioxidant action, indicating that it could be useful as an adjunctive therapy for the treatment of Parkinson's disease.
本研究旨在探讨儿茶素在帕金森病模型中的有益作用。单侧纹状体 6-羟多巴胺（6-OHDA）损伤大鼠腹腔注射儿茶素（10 和 30mg/kg）预处理，于术前 2 小时及术后 14 天进行。治疗后，评价阿扑吗啡诱导的旋转、运动活性、工作记忆以及早期和晚期厌恶记忆。使用中脑测定单胺水平和谷胱甘肽（GSH）的测量。免疫组织化学染色也用于评估中脑和纹状体组织中酪氨酸羟化酶（TH）的表达。儿茶素给药减轻了损伤大鼠观察到的旋转行为增加和运动活性下降。虽然儿茶素不能挽救晚期厌恶记忆的损伤，但它能保护动物免受 6-OHDA 诱导的工作记忆缺陷。此外，儿茶素治疗恢复了 GSH 水平，并显著增加了 6-OHDA 损伤大鼠中多巴胺和 DOPAC 的含量以及 TH 免疫反应性。儿茶素通过抗氧化作用保护 6-OHDA 损伤大鼠，表明其可用作治疗帕金森病的辅助治疗。
