Gentili G, Wirz M, Collotti C, Vicari G, Sernicola L, Verani P, Pizzocolo G, Crovari P, Icardi G, Ortona L
Laboratorio di Immunologia, Istituto Superiore di Sanità, Roma.
Ric Clin Lab. 1990 Apr-Jun;20(2):95-103. doi: 10.1007/BF02877555.
This paper reports the results of a study organized by the Istituto Superiore di Sanità (Rome, Italy) and carried out in collaboration with several national laboratories. Its aim was to ascertain whether diagnostic kits for detecting anti-HIV antibodies in human serum can also be used to detect the same antibodies in human immunoglobulins. Thirty-three lots of immunoglobulins supplied by six pharmaceutical companies present in Italy were examined using different procedures. On the basis of the results of this study it can be concluded that i. anti-HIV antibodies can be detected in immunoglobulins by means of commercial reagents; ii. a preliminary dilution of immunoglobulin samples should not be made; iii. Western blot method appears to be the 'reference' test; however, competitive EIA tests are equally valuable if a negative control made up of anti-HIV-negative Igs is employed; iv. Igs examination for anti-HIV antibody represents an indirect control on a correct donors' screening procedure.
本文报告了一项由意大利罗马高级卫生研究院组织并与多个国家实验室合作开展的研究结果。其目的是确定用于检测人血清中抗HIV抗体的诊断试剂盒是否也可用于检测人免疫球蛋白中的相同抗体。使用不同程序对意大利六家制药公司提供的33批免疫球蛋白进行了检测。根据本研究结果可以得出以下结论：i. 可通过商业试剂在免疫球蛋白中检测到抗HIV抗体；ii. 不应预先稀释免疫球蛋白样本；iii. 蛋白质印迹法似乎是“参考”检测方法；然而，如果采用由抗HIV阴性免疫球蛋白组成的阴性对照，则竞争性酶免疫测定同样有价值；iv. 检测免疫球蛋白中的抗HIV抗体是对正确的供血者筛查程序的间接控制。
