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[用于测定抗疱疹制剂比活性的商业酶免疫分析测试系统的评估]

[The evaluation of a commercial EIA test system for determining the specific activity of antiherpetic preparations].

作者信息

Borsuk E A, Vorob'eva M S, Ebralidze L K

出版信息

Vopr Virusol. 1991 Mar-Apr;36(2):137-40.

PMID:1652869
Abstract

The data on the use of a commercial EIA test system for detection of antibodies in control of preparations against herpes simplex and cytomegaloviruses are presented. The enzyme immunoassay test system for antibody determinations to herpes simplex virus produced by the Odessa bacterial preparations enterprise was shown to be suitable for determination of the specific potency (antigenicity) of herpes simplex vaccine. The advantages of this method over the currently used neutralization test were established. Titration of commercial immunoglobulins detects lots with high litres of antibody to herpes simplex virus. For the same purpose, lots of commercial immunoglobulins were tested for antibodies to cytomegalovirus using a West Germany test-system (Behring). It is concluded that enzyme immunoassay test systems for antibody determinations may be used for screening of lots of immunoglobulins of special effects (against herpes simplex and cytomegalovirus infections) both at the stage of serum and final preparation screening.

摘要

本文介绍了在检测针对单纯疱疹病毒和巨细胞病毒制剂的对照中使用商用酶免疫分析(EIA)测试系统检测抗体的数据。敖德萨细菌制剂企业生产的用于检测单纯疱疹病毒抗体的酶免疫分析测试系统被证明适用于测定单纯疱疹疫苗的比活性(抗原性)。确定了该方法相对于目前使用的中和试验的优势。滴定商用免疫球蛋白可检测出对单纯疱疹病毒抗体效价高的批次。出于同样目的,使用西德测试系统(贝林)检测了许多商用免疫球蛋白针对巨细胞病毒的抗体。得出的结论是,用于抗体测定的酶免疫分析测试系统可用于在血清和最终制剂筛选阶段筛选具有特效(针对单纯疱疹和巨细胞病毒感染)的免疫球蛋白批次。

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