Díaz Miguel Ángel, Díaz Paula Lucía, Rodríguez Edna Catering, Montaño Lucy Angeline, Gartner Doris Mabel, Vernaza María Elisa, Eljach Victoria, Realpe María Elena
Grupo de Microbiología, Subdirección Red Nacional de Laboratorios, Instituto Nacional de Salud, Bogotá, D.C., Colombia.
Biomedica. 2013 Jan-Mar;33(1):62-9. doi: 10.1590/S0120-41572013000100008.
Salmonella Enteritidis is recognized worldwide as one of the main agents of human gastrointestinal infection. Several reports indicate the presence of isolates with decreased sensitivity to ciprofloxacin that can lead to a delayed response or the development of resistance during treatment.
To describe and characterize isolates of Salmonella Enteritidis associated to an outbreak of food-borne diseases in Popayán, Cauca.
Ten Salmonella Enteritidis isolates from nine patients and one food sample (chicken sandwich) were analyzed by biochemical tests, serotyping and antimicrobial sensitivity. The minimum inhibitory concentration to ciprofloxacin was determined by E-test and the genetic profile of the isolates was tested by pulsed field gel electrophoresis (PFGE) with XbaI and Blnl enzymes.
Salmonella Enteritidis was identified in all isolates. They were resistant to nalidixic acid and had a decreased sensitivity to ciprofloxaxin between 0.25 and 0.5 μg/ml; all isolates were sensitive to all the other antimicrobials we tested. Ten isolates were grouped by PFGE with the XbaI enzyme in the COIN11.JEG.X01.0038 pattern, and seven isolates were confirmed with the BlnI enzyme using the COIN11.JEG.A26.0009 pattern.
We report for the first time an outbreak of nalidixic acid-resistant Salmonella Enteritidis in Colombia and confirmed by phenotypic and genotypic analysis the association between the isolates from patients and the chicken sandwich as the source of infection.
肠炎沙门氏菌是全球公认的人类胃肠道感染的主要病原体之一。多项报告表明，存在对环丙沙星敏感性降低的分离株，这可能导致治疗期间反应延迟或产生耐药性。
描述并鉴定与考卡省波帕扬食源性疾病暴发相关的肠炎沙门氏菌分离株。
对来自9名患者和1份食品样本（鸡肉三明治）的10株肠炎沙门氏菌分离株进行生化试验、血清分型和药敏分析。采用E-test法测定对环丙沙星的最低抑菌浓度，并用XbaI和BlnI酶通过脉冲场凝胶电泳（PFGE）检测分离株的基因图谱。
所有分离株均鉴定为肠炎沙门氏菌。它们对萘啶酸耐药，对环丙沙星的敏感性在0.25至0.5μg/ml之间降低；所有分离株对我们测试的所有其他抗菌药物均敏感。10株分离株用XbaI酶通过PFGE分为COIN11.JEG.X01.0038型，7株分离株用BlnI酶确认为COIN11.JEG.A26.0009型。
我们首次报告了哥伦比亚耐萘啶酸肠炎沙门氏菌的暴发，并通过表型和基因型分析证实了患者分离株与作为感染源的鸡肉三明治之间的关联。
