Department of Chemistry, School of Science, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
Biol Trace Elem Res. 2013 Jul;154(1):150-5. doi: 10.1007/s12011-013-9707-7. Epub 2013 May 29.
The antibacterial mechanisms of Cu(phen)2Cl2·6H2O, [Cu(phen)(Gly)(H2O)]Cl·3H2O, [Cu(phen)(L-Ser)(H2O)Cl] (1,10-phenanthroline (phen)) on Escherichia coli were investigated. In the inductively coupled plasma atomic emission spectroscopy experiments, it showed that lipophilic phen ligand can cause elevation of intracellular copper, but intracellular copper is not the decisive factor. The UV-vis and gel electrophoresis experiments reveal that the DNA binding and cleavage activity are decisive factors for the antibacterial action of these compounds. It is revealed by the cyclic voltammetry experiments that the redox potential was bound to the cleave activity.
研究了 Cu(phen)2Cl2·6H2O、[Cu(phen)(Gly)(H2O)]Cl·3H2O、[Cu(phen)(L-Ser)(H2O)Cl]（1,10-菲咯啉（phen））对大肠杆菌的抗菌机制。在电感耦合等离子体原子发射光谱实验中，结果表明亲脂性 phen 配体能引起细胞内铜的升高，但细胞内铜不是决定因素。紫外可见和凝胶电泳实验表明，DNA 结合和切割活性是这些化合物抗菌作用的决定性因素。循环伏安实验表明，氧化还原电位与切割活性有关。
