Hôpital du Savoré-Coeur de Montréal, Université de Montréal, Montréal, Québec.
Can Respir J. 2013 Jul-Aug;20(4):248-52. doi: 10.1155/2013/591316. Epub 2013 May 28.
The use of inflammometry has been shown to be effective for managing asthma. However, sputum processing can be time consuming. Furthermore, methods of sputum processing can vary among facilities. To help with standardization and to simplify the procedure for laboratory staff, a novel, commercially available processing device (Accufilter, Cellometrics Inc, Canada) has been developed.
To assess the validity of the Accufilter device and kit for recovery of treated specimens, and for quantitative sputum inflammatory cell counts by comparing intrasample measurements with those using the same procedure without the Accufilter device and kit.
The present study was a wet laboratory comparison of induced sputum cell counts obtained from sputum processed with versus without the device and kit. Comparisons of each sputum specimen were performed by the same technologist in random order.
A total of 39 samples were processed using both the standard method and the Accufilter device. The intraclass correlation coefficients were high for the weight of the filtrate, and for eosinophil and neutrophil differential counts.
A good degree of agreement of results was apparent when the two methods were compared. The differences noted between both methods were minimal and did not modify clinical interpretation. The use of the Accufilter device and kit can be used in place of the standard method for sputum quantitative analysis, especially in centres with large sample loads.
炎症测定法已被证明可有效管理哮喘。然而，痰液处理可能很耗时。此外，痰液处理方法在不同机构之间可能存在差异。为了帮助标准化并简化实验室人员的操作程序，开发了一种新型的、市售的处理设备（Accufilter，Cellometrics Inc，加拿大）。
通过比较使用和不使用 Accufilter 设备和试剂盒处理的相同样本的内样本测量值与定量痰液炎症细胞计数，评估 Accufilter 设备和试剂盒对处理标本的回收率和定量痰液炎症细胞计数的有效性。
本研究是对使用和不使用设备和试剂盒处理的诱导痰液细胞计数进行的湿实验室比较。每个痰液标本的比较都是由同一位技术人员随机顺序进行的。
共有 39 个样本同时使用标准方法和 Accufilter 设备进行处理。滤出物重量和嗜酸性粒细胞和中性粒细胞差异计数的组内相关系数均较高。
当比较两种方法时，明显出现了结果高度一致的情况。两种方法之间的差异很小，不会改变临床解释。Accufilter 设备和试剂盒的使用可以替代痰液定量分析的标准方法，尤其是在样本负荷较大的中心。
