Valentin A, Albert J, Fenyö E M, Asjö B
Department of Virology, Karolinska Institute, Stockholm, Sweden.
Virology. 1990 Aug;177(2):790-4. doi: 10.1016/0042-6822(90)90551-2.
We have investigated the replicative capacity of 14 primary HIV-1 isolates in cultures of normal blood macrophages and PHA-stimulated peripheral blood mononuclear cells (PBMC). All viruses could infect normal macrophages as demonstrated by either reverse transcriptase (RT) activity, p24 antigen assay, or cocultivation with PBMC. One month after infection of macrophages virus could no longer be detected in the culture medium. The cells remained in this nonproductive state for another month. Virus could, however, be recovered by cocultivation with PHA-stimulated PBMC. Such macrophage-passaged virus induced pronounced cell killing with fragmentation and pyknosis and often replicated poorly in PBMC, in contrast to the original isolate. The results indicate that all primary HIV-1 isolates contain virus variants that can infect cells of the monocyte/macrophage lineage. Persistently infected, seemingly nonproducing cells, may serve as infectious reservoirs in the infected individual and spread infection to other susceptible cells over a long period of time. Moreover, the pronounced killing of PBMC by the macrophage-harbored virus may contribute to the deterioration of the immune system.
我们研究了14株原发性HIV-1分离株在正常血液巨噬细胞和PHA刺激的外周血单核细胞（PBMC）培养物中的复制能力。通过逆转录酶（RT）活性、p24抗原检测或与PBMC共培养证明，所有病毒均可感染正常巨噬细胞。巨噬细胞感染病毒1个月后，培养基中不再能检测到病毒。细胞在这种非生产性状态下持续1个月。然而，通过与PHA刺激的PBMC共培养可回收病毒。与原始分离株相比，这种经巨噬细胞传代的病毒可导致明显的细胞杀伤，出现细胞破碎和固缩，且在PBMC中通常复制不佳。结果表明，所有原发性HIV-1分离株均包含可感染单核细胞/巨噬细胞谱系细胞的病毒变体。持续感染、看似不产生病毒的细胞可能作为感染个体中的感染储存库，并在很长一段时间内将感染传播给其他易感细胞。此外，巨噬细胞携带的病毒对PBMC的明显杀伤可能导致免疫系统恶化。
