Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China.
Clin Microbiol Infect. 2013 Aug;19(8):E372-5. doi: 10.1111/1469-0691.12263. Epub 2013 May 30.
A rapid and sensitive H7 and N9 subtype-specific reverse transcription loop-mediated isothermal amplification assay was developed respectively for visual detection of human-infected influenza A (H7N9) virus. The reaction was performed in one step in a single tube at 63°C for 60 min with the addition of hydroxynaphthol blue dye before amplification. The detection limits of both subtype-specific assays were comparable to those of validated H7 and N9 real-time PCR assays respectively and no cross-detection was observed with influenza A pandemic H1N1, H3N2, H5N1, H9N2 or influenza B virus. The assays were evaluated further with H7N9 virus-infected clinical specimens.
分别建立了快速、灵敏的 H7 和 N9 亚型特异性逆转录环介导等温扩增检测方法，用于直观检测人感染的甲型流感（H7N9）病毒。该反应在 63°C 下一步进行，在扩增前加入羟基萘酚蓝染料，在 60 分钟内完成。两种亚型特异性检测方法的检测限分别与已验证的 H7 和 N9 实时 PCR 检测方法相当，与甲型流感大流行 H1N1、H3N2、H5N1、H9N2 或乙型流感病毒无交叉检测。进一步用 H7N9 病毒感染的临床标本评估了这些检测方法。
