用于快速诊断甲型禽流感（H7N9）病毒感染的逆转录环介导等温扩增检测方法的建立。
Development of a reverse transcription loop-mediated isothermal amplification assay for the rapid diagnosis of avian influenza A (H7N9) virus infection.
作者信息
Nakauchi Mina, Takayama Ikuyo, Takahashi Hitoshi, Tashiro Masato, Kageyama Tsutomu
机构信息
Influenza Virus Research Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
Influenza Virus Research Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
出版信息
J Virol Methods. 2014 Aug;204:101-4. doi: 10.1016/j.jviromet.2014.03.028. Epub 2014 Apr 18.
A genetic diagnosis system for detecting avian influenza A (H7N9) virus infection using reverse transcription-loop-mediated isothermal amplification (RT-LAMP) technology was developed. The RT-LAMP assay showed no cross-reactivity with seasonal influenza A (H3N2 and H1N1pdm09) or influenza B viruses circulating in humans or with avian influenza A (H5N1) viruses. The sensitivity of the RT-LAMP assay was 42.47 copies/reaction. Considering the high specificity and sensitivity of the assay for detecting the avian influenza A (H7N9) virus and that the reaction was completed within 30 min, the RT-LAMP assay developed in this study is a promising rapid diagnostic tool for avian influenza A (H7N9) virus infection.
开发了一种利用逆转录环介导等温扩增（RT-LAMP）技术检测甲型H7N9禽流感病毒感染的基因诊断系统。该RT-LAMP检测方法与在人类中传播的季节性甲型流感（H3N2和H1N1pdm09）或乙型流感病毒以及甲型H5N1禽流感病毒均无交叉反应。RT-LAMP检测方法的灵敏度为42.47拷贝/反应。鉴于该检测方法在检测甲型H7N9禽流感病毒方面具有高特异性和灵敏度，且反应在30分钟内完成，本研究开发的RT-LAMP检测方法是一种用于甲型H7N9禽流感病毒感染的有前景的快速诊断工具。
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