从培养的肝癌细胞中分离细胞外纳米囊泡微小RNA
Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture.
作者信息
Kogure Takayuki, Patel Tushar
机构信息
Mayo Clinic, Jacksonville, FL, USA.
出版信息
Methods Mol Biol. 2013;1024:11-8. doi: 10.1007/978-1-62703-453-1_2.
PMID:23719939
Abstract
MicroRNA can be transferred across cells within extracellular vesicles such as exosomes. In order to analyze the biological effects of extracellular vesicle microRNA, it is necessary to isolate these vesicles and to extract their miRNA content.Here, we describe an approach to the isolation of cellular nanovesicles from liver cancer cell lines that can be used for the isolation of RNA and microRNA.
摘要
微小RNA可以通过细胞外囊泡（如外泌体）在细胞间转移。为了分析细胞外囊泡微小RNA的生物学效应，有必要分离这些囊泡并提取其微小RNA含量。在此，我们描述了一种从肝癌细胞系中分离细胞纳米囊泡的方法，该方法可用于RNA和微小RNA的分离。
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