Wolf Michael, Lossdörfer Stefan, Küpper Katharina, Jäger Andreas
*Department of Orthodontics, Dental Clinic, University of Bonn and
*Department of Orthodontics, Dental Clinic, University of Bonn and.
Eur J Orthod. 2014 Dec;36(6):624-31. doi: 10.1093/ejo/cjt037. Epub 2013 May 29.
The chromatin-binding protein high mobility group box protein 1 (HMGB1) can be released into the extracellular milieu by necrotic and damaged cells and functions as an alarmin that is recognized by the innate immune system to initiate and modulate tissue repair. However, little is known about the function of HMGB1 within orthodontic tooth movement. Therefore, it was the aim of the present study to investigate HMGB1 expression by periodontal ligament (PDL) cells challenged by mechanical loading similar to force levels being applied in orthodontic treatment in vitro and to transfer these findings to an in vivo microenvironment in an animal model of tooth movement in rats. In addition, we addressed the question of whether the regulation of HMGB1 expression is modulated in a time-dependent manner in zones of compressive forces.
Protein analysis revealed a basal HMGB1 expression in PDL cell cultures and an increased expression when orthodontic forces were applied. In a rat model of tooth movement including 25 animals that received orthodontic tooth movement for 3, 6, 9, and 12 days, HMGB1 protein expression was demonstrated to be regulated in a time-dependent manner as determined by means of immunohistochemistry and histomorphometrical analyses.
These data indicate a potential role for HMGB1 protein originating from PDL cells in the regulation of orthodontic tooth movement and the periodontal remodelling process by modifying the local microenvironment.
摘要 引言：染色质结合蛋白高迁移率族蛋白B1（HMGB1）可由坏死和受损细胞释放到细胞外环境中，并作为一种警报素发挥作用，被先天免疫系统识别以启动和调节组织修复。然而，关于HMGB1在正畸牙齿移动中的功能知之甚少。因此，本研究的目的是在体外研究牙周膜（PDL）细胞在类似于正畸治疗中所施加力水平的机械加载刺激下HMGB1的表达情况，并将这些发现应用于大鼠牙齿移动动物模型的体内微环境。此外，我们还探讨了在压力区域中HMGB1表达的调节是否以时间依赖性方式进行。
蛋白质分析显示，PDL细胞培养物中有基础HMGB1表达，施加正畸力时表达增加。在一个包含25只接受正畸牙齿移动3、6、9和12天的动物的大鼠牙齿移动模型中，通过免疫组织化学和组织形态计量学分析确定，HMGB1蛋白表达以时间依赖性方式受到调节。
这些数据表明，源自PDL细胞的HMGB1蛋白通过改变局部微环境，在正畸牙齿移动和牙周重塑过程的调节中可能发挥作用。
