Lv Shengyu, Li Juan, Feng Wei, Liu Hongrui, Du Juan, Sun Jing, Cui Jian, Sun Bao, Han Xiuchun, Oda Kimimitsu, Amizuka Norio, Xu Xin, Li Minqi
Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Bone Metabolism, School of Stomatology, Shandong University, Wenhua West Road 44-1, Jinan, 250012, China.
J Mol Histol. 2015 Feb;46(1):107-14. doi: 10.1007/s10735-014-9606-z. Epub 2014 Dec 19.
Recent studies indicate that high mobility group box protein 1 (HMGB1) originating from periodontal ligament (PDL) cells can be a potential regulator in the process of orthodontic tooth movement and periodontal tissue remodeling. The aim of this study is to investigate HMGB1 expression in periodontal tissue during orthodontic tooth movement in mice according to Waldo's method. Six 7-week-old C57BL6 mice were used in these experiments. The elastic band was inserted into the teeth space between the right first and second maxillary molars. After 3 days of mechanical loading, mice were fixed with transcardial perfusion of 4 % paraformaldehyde in 0.1 M phosphate buffer (pH 7.4), and the maxillary was extracted for histochemical analyses. The histological examination revealed local PDL tear at the tension side and the formation of extensive cell-free hyaline zones at the compression side. The immunolocalization of HMGB1 was significantly presented at tension side of PDL, apical area and dental pulp, whereas at the compression side of PDL, the labeling of HMGB1 was almost undetectable as the presence of hyaline zone. Taken together, we concluded that the orthodontic tooth movement by Waldo's method leads to histological changes and HMGB1 expression pattern that differ from those of coil spring method, including PDL tear and extensive hyaline zone which may severely destroy periodontal tissue and in turn impede tooth movement.
近期研究表明，源自牙周膜（PDL）细胞的高迁移率族蛋白B1（HMGB1）可能是正畸牙齿移动和牙周组织重塑过程中的潜在调节因子。本研究的目的是根据沃尔多方法，研究小鼠正畸牙齿移动过程中牙周组织中HMGB1的表达情况。这些实验使用了6只7周龄的C57BL6小鼠。将橡皮筋插入右侧上颌第一和第二磨牙之间的牙间隙。机械加载3天后，通过在0.1M磷酸盐缓冲液（pH 7.4）中经心内灌注4%多聚甲醛对小鼠进行固定，然后取出上颌进行组织化学分析。组织学检查显示，张力侧局部牙周膜撕裂，压缩侧形成广泛的无细胞透明带。HMGB1的免疫定位在牙周膜张力侧、根尖区和牙髓中显著呈现，而在牙周膜压缩侧，由于透明带的存在，几乎检测不到HMGB1的标记。综上所述，我们得出结论，沃尔多方法进行的正畸牙齿移动导致的组织学变化和HMGB1表达模式与螺旋弹簧法不同，包括牙周膜撕裂和广泛的透明带，这可能会严重破坏牙周组织，进而阻碍牙齿移动。
