Da'at Arina Yuliana Mahdiyah, Rubianto Mohamad, Ferdiansyah F, Sudiana I Ketut, Rahayu Retno Pudji, Notobroto Hari Basuki
Department of Periodontology, Faculty of Dentistry, Jember University, Jember, Indonesia.
Department of Periodontology, Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia.
Contemp Clin Dent. 2019 Jul-Sep;10(3):525-530. doi: 10.4103/ccd.ccd_907_18.
High-mobility group box 1 (HMGB1) was suggested to be associated with the pathogenesis of chronic periodontitis which characterized by alveolar bone loss. HMGB1 was defined as a bone-active cytokine, but the rule of HMGB1 in bone loss of chronic periodontitis is still understood.
The aim of this study is to investigate the expression of HMGB1 on osteoblasts and osteoclasts in the mandible of chronic periodontitis.
This experimental study was conducted to rats injected by into the buccal and lingual subgingival area at a concentration of 2 × 10 CFU/mL three times a week with 2-day apart for 2, 3, 4, and 6 weeks as chronic periodontitis group and injected by normal saline as control group. Analysis of variance was used to examine the differences between groups followed by least significant difference test with the level of significance was <0.05.
The HMGB1 expression was found in both osteoclasts and osteoblasts of mandibular bone by immunohistochemistry analysis. There was a difference of HMGB1 expression on osteoblasts and osteoclasts of chronic periodontitis. HMGB1 expression was found increased significantly in mandibular osteoblasts of chronic periodontitis, whereas the HMGB1 expression in mandibular osteoclast is higher in 2 and 3 weeks, but it was lower in 4 and 6 weeks.
This study indicated a potential role for HMGB1 in bone loss of chronic periodontitis. HMGB1 on mandibular osteoclasts and osteoblasts may play different rules in the onset and progression of chronic periodontitis.
高迁移率族蛋白B1（HMGB1）被认为与以牙槽骨吸收为特征的慢性牙周炎的发病机制有关。HMGB1被定义为一种骨活性细胞因子，但HMGB1在慢性牙周炎骨吸收中的作用仍不清楚。
本研究旨在探讨慢性牙周炎大鼠下颌骨中HMGB1在成骨细胞和破骨细胞上的表达。
本实验研究将大鼠分为慢性牙周炎组和对照组，慢性牙周炎组大鼠每周3次在颊侧和舌侧龈下区域注射浓度为2×10CFU/mL的细菌，间隔2天，分别注射2、3、4和6周；对照组大鼠注射生理盐水。采用方差分析检验组间差异，随后进行最小显著差检验，显著性水平为<0.05。
通过免疫组织化学分析发现下颌骨的破骨细胞和成骨细胞中均有HMGB1表达。慢性牙周炎的成骨细胞和破骨细胞上HMGB1表达存在差异。慢性牙周炎下颌骨成骨细胞中HMGB1表达显著增加，而慢性牙周炎下颌骨破骨细胞中HMGB1表达在2周和3周时较高，但在4周和6周时较低。
本研究表明HMGB1在慢性牙周炎骨吸收中具有潜在作用。下颌骨破骨细胞和成骨细胞上的HMGB1在慢性牙周炎的发生和发展中可能发挥不同作用。
