Boretskaia M A, Suslova O S
Mikrobiol Z. 2013 Mar-Apr;75(2):45-9.
The optimal methods for the extraction of exopolymeric complex (EPS) of Stenotrophomonas maltophilia 22M was determined. That EPS was synthesized in plankton and biofilm growth mode on the mild steel surface. It is desirable to use different physical and chemical methods for studying the EPS composition (carbohydrates and proteins) depending on the bacteria growth mode. In this way the interaction with ion exchange resin was the most effective for plankton growth mode to determine the maximum amount of carbohydrates (9.5 microg/ml), and the impact of heating to determine protein (3.9 microg/ml). For EPS biofilm in order to obtain maximum amount of carbohydrate it is desirable to use heating (30 microg/ml) and centrifugation (35 microg/ml). It is recommended to determine protein in the biofilm EPS after treatment with heating (3.75 microg/ml) and centrifugation (3.75 microg/ml).
确定了嗜麦芽窄食单胞菌22M胞外聚合物复合体（EPS）的最佳提取方法。该EPS在浮游和生物膜生长模式下于低碳钢表面合成。根据细菌生长模式，使用不同的物理和化学方法研究EPS组成（碳水化合物和蛋白质）是很有必要的。这样，与离子交换树脂的相互作用对浮游生长模式最有效，可确定碳水化合物的最大量（9.5微克/毫升），加热对确定蛋白质（3.9微克/毫升）有影响。对于EPS生物膜，为获得最大量的碳水化合物，宜采用加热（30微克/毫升）和离心（35微克/毫升）。建议在加热（3.75微克/毫升）和离心（3.75微克/毫升）处理后测定生物膜EPS中的蛋白质。
