Churkina L N, Avdeeva L V, Voĭchuk S I, Iaroshenko L V
Mikrobiol Z. 2013 Mar-Apr;75(2):67-71.
Variability of properties and antibiotic activity, as well as cells survival of Pseudomonas batumici 17/20--the producer of batumin (antistaphylococcal antibiotic) after long-term storage under vaseline oil layer have been studied. The main culture-morphological and physiological biochemical properties of the mutant strain have been investigated. It has been shown that storage under vaseline oil allows to preserve high level of antibiotic activity: batumin synthesis by the producer was 150 mg/l. Therewith, the survival of cells decreases by two orders during 5 years of storage. The conditions of strain maintenance have been formulated.
研究了巴氏假单胞菌17/20(杆菌肽(抗葡萄球菌抗生素)的产生菌)在凡士林油层下长期保存后其特性、抗生素活性以及细胞存活率的变异性。对突变菌株的主要培养形态学和生理生化特性进行了研究。结果表明,在凡士林油下保存可保持较高水平的抗生素活性:产生菌合成的杆菌肽为150毫克/升。与此同时,在保存5年期间细胞存活率下降了两个数量级。制定了菌株保存条件。