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[八丈链霉菌(口古uchi)巴尔达奇(Streptoverticillium hachijoense (Jamaguchi) Baldacci)长期实验室保存的结果,曲古霉素的产生菌]

[Results of the long laboratory storage of Streptoverticillium hachijoense (Jamaguchi) Baldacci, the producer of trichomycin].

作者信息

Konev Iu E, Kuz'mina E D

出版信息

Antibiotiki. 1975 Mar;20(3):235-9.

PMID:1169905
Abstract

Viability, morphologo-cultural features and antibiotic properties of Sv. hachijoense, strain LIA-0052 stored for 10 years in a dry state and in the state of a resting culture were studied. Spores and mycelium of 2-week strains most stable to some chemical and physical factors were used for drying. It was found that viability of strain LIA-0052 was maintained for a longer period of time after lyophilization, in garden soil and agar culture under a layer of mineral oil. By the end of the observation period the viability of the soil culture decreased and the morphologo-cultural properties were stabilized. When the strain was cultivated on media with sucrose, the level of its antibiotic activity increased.

摘要

研究了在干燥状态和静止培养状态下保存10年的八丈岛链霉菌(Streptomyces hachijoense)LIA-0052菌株的活力、形态培养特征和抗生素特性。使用对某些化学和物理因素最稳定的2周龄菌株的孢子和菌丝体进行干燥处理。结果发现,LIA-0052菌株在冻干后、在花园土壤中以及在矿物油层下的琼脂培养中,其活力能维持较长时间。到观察期末,土壤培养物的活力下降,形态培养特性趋于稳定。当该菌株在含蔗糖的培养基上培养时,其抗生素活性水平会提高。

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