Konev Iu E, Kuz'mina E D
Antibiotiki. 1975 Mar;20(3):235-9.
Viability, morphologo-cultural features and antibiotic properties of Sv. hachijoense, strain LIA-0052 stored for 10 years in a dry state and in the state of a resting culture were studied. Spores and mycelium of 2-week strains most stable to some chemical and physical factors were used for drying. It was found that viability of strain LIA-0052 was maintained for a longer period of time after lyophilization, in garden soil and agar culture under a layer of mineral oil. By the end of the observation period the viability of the soil culture decreased and the morphologo-cultural properties were stabilized. When the strain was cultivated on media with sucrose, the level of its antibiotic activity increased.
研究了在干燥状态和静止培养状态下保存10年的八丈岛链霉菌(Streptomyces hachijoense)LIA-0052菌株的活力、形态培养特征和抗生素特性。使用对某些化学和物理因素最稳定的2周龄菌株的孢子和菌丝体进行干燥处理。结果发现,LIA-0052菌株在冻干后、在花园土壤中以及在矿物油层下的琼脂培养中,其活力能维持较长时间。到观察期末,土壤培养物的活力下降,形态培养特性趋于稳定。当该菌株在含蔗糖的培养基上培养时,其抗生素活性水平会提高。