Himani G S, Prabhuji M L V, Karthikeyan B V
Department of Periodontics, MGM Dental College and Hospital, Navi Mumbai, Maharashtra, India.
J Periodontal Res. 2014 Apr;49(2):237-45. doi: 10.1111/jre.12100. Epub 2013 May 31.
There is a substantial magnitude of data implicating the role of interleukin-23 (IL-23) in the initiation and progression of periodontal disease. The main aim of this study was to evaluate the levels of IL-23 in the gingival crevicular fluid of systemically healthy subjects in periodontal health and disease. In addition, we explored the effectiveness of periodontal interventional therapy on the levels of IL-23 in subjects with chronic periodontitis to obtain a deeper insight into the possible role of IL-23 in three separate periodontal conditions in three different populations.
In this study, 54 individuals, satisfying the study inclusion and exclusion criteria, were recruited. They were categorically divided, on the basis of gingival index, probing pocket depth and relative attachment loss, into three groups: Group 1 (patients with a clinically healthy periodontium, n = 18); Group 2 (patients with gingivitis, n = 18); and Group 3a (patients with chronic periodontitis, n = 18). Samples taken from all 18 subjects of Group 3a, 3 mo after the initial therapy, constituted Group 3b. All clinical parameters were recorded at baseline and 3 mo after scaling and root planing. Gingival crevicular fluid samples were obtained in which the IL-23 concentration was measured using ELISA.
The highest mean IL-23 concentration in gingival crevicular fluid was found for Group 3a (16448.69 pg/mL) and the lowest for Group 1 (2565.28 pg/mL). The mean IL-23 concentrations in Group 2 (5425 pg/mL) and Group 3b (6272.22 pg/mL) lay between the maximum and minimum values. This implies a positive correlation between the gingival crevicular fluid IL-23 concentration and relative attachment loss (p < 0.05).
A noteworthy increase in the gingival crevicular fluid IL-23 concentration was seen that was proportional to the amount of periodontal tissue damage. As the IL-23 concentration in gingival crevicular fluid is directly proportional to the severity of the periodontal affliction, it can be speculated that IL-23 has a possible role in the pathogenesis of periodontal disease.
有大量数据表明白细胞介素-23（IL-23）在牙周病的发生和发展中起作用。本研究的主要目的是评估全身健康的受试者在牙周健康和患病状态下龈沟液中IL-23的水平。此外，我们探讨了牙周介入治疗对慢性牙周炎患者IL-23水平的影响，以更深入地了解IL-23在三种不同人群的三种不同牙周状况中的可能作用。
本研究招募了54名符合研究纳入和排除标准的个体。根据牙龈指数、探诊深度和相对附着丧失情况，将他们明确分为三组：第1组（临床牙周健康患者，n = 18）；第2组（牙龈炎患者，n = 18）；第3a组（慢性牙周炎患者，n = 18）。第3a组的所有18名受试者在初始治疗3个月后采集的样本构成第3b组。在基线以及龈上洁治和根面平整后3个月记录所有临床参数。采集龈沟液样本，使用酶联免疫吸附测定法（ELISA）测量其中IL-23的浓度。
第3a组龈沟液中IL-23的平均浓度最高（16448.69 pg/mL），第1组最低（2565.28 pg/mL）。第2组（5425 pg/mL）和第3b组（6272.22 pg/mL）的平均IL-23浓度介于最大值和最小值之间。这表明龈沟液IL-23浓度与相对附着丧失之间存在正相关（p < 0.05）。
观察到龈沟液中IL-23浓度显著升高，且与牙周组织损伤程度成正比。由于龈沟液中IL-23浓度与牙周疾病的严重程度直接相关，可以推测IL-23在牙周病发病机制中可能起作用。
