Pradeep A R, Raj S, Aruna G, Chowdhry S
Department of Periodontics, Government Dental College and Hospital, Bangalore, Karnataka, India.
J Periodontal Res. 2009 Apr;44(2):232-7. doi: 10.1111/j.1600-0765.2008.01138.x. Epub 2008 Oct 7.
The level of Substance-P in gingival crevicular fluid has been found to correlate with clinical measures of periodontal disease. The present study was designed to assess the relationship between clinical parameters and levels of Substance-P in the gingival crevicular fluid from inflamed gingiva, periodontitis sites and after treatment of periodontitis sites, and to correlate them to the Substance-P levels of plasma.
Thirty, age- and gender-matched subjects were divided into three groups (healthy, gingivitis and chronic periodontitis) based on modified gingival index scores and clinical attachment loss. A fourth group consisted of 10 subjects from the periodontitis group, 6-8 wk after initial therapy. Plasma and gingival crevicular fluid samples were collected and quantified for Substance-P using an enzyme immunoassay.
The mean concentration of Substance-P, both in gingival crevicular fluid and plasma, was observed to be highest in the periodontitis group (45.13 pg/mL in gingival crevicular fluid and 67.8 pg/mL in plasma) and lowest in the healthy group (6.07 pg/mL in gingival crevicular fluid and below the detection level in plasma). The mean Substance-P concentration in the gingivitis group (11.42 pg/mL in gingival crevicular fluid and 38.8 pg/mL in plasma) and in the after-treatment group (7.58 pg/mL in gingival crevicular fluid and 39.7 pg/mL in plasma) lay between the highest and lowest values. In all groups the gingival crevicular fluid levels showed a statistically significant positive correlation with that of plasma and clinical attachment loss.
Substance-P levels were highest in the gingival crevicular fluid from sites with periodontal destruction; however, periodontal treatment resulted in the reduction of Substance-P levels. Gingival crevicular fluid and plasma Substance-P levels showed a positive correlation in all of the groups.
已发现龈沟液中P物质的水平与牙周疾病的临床指标相关。本研究旨在评估临床参数与来自炎症牙龈、牙周炎部位及牙周炎部位治疗后的龈沟液中P物质水平之间的关系，并将它们与血浆中P物质的水平相关联。
根据改良牙龈指数评分和临床附着丧失情况，将30名年龄和性别匹配的受试者分为三组（健康组、牙龈炎组和慢性牙周炎组）。第四组由10名来自牙周炎组且在初始治疗后6 - 8周的受试者组成。采集血浆和龈沟液样本，并使用酶免疫测定法定量P物质。
观察到牙周炎组龈沟液和血浆中P物质的平均浓度最高（龈沟液中为45.13 pg/mL，血浆中为67.8 pg/mL），健康组最低（龈沟液中为6.07 pg/mL，血浆中低于检测水平）。牙龈炎组（龈沟液中为11.42 pg/mL，血浆中为38.8 pg/mL）和治疗后组（龈沟液中为7.58 pg/mL，血浆中为39.7 pg/mL）的P物质平均浓度介于最高值和最低值之间。在所有组中，龈沟液水平与血浆水平及临床附着丧失均呈统计学显著正相关。
牙周破坏部位的龈沟液中P物质水平最高；然而，牙周治疗导致P物质水平降低。所有组中龈沟液和血浆P物质水平均呈正相关。
