Department of Chemistry, University of Pennsylvania, 231 S. 34th Street, Philadelphia, PA 19104, USA.
Bioorg Med Chem. 2013 Oct 15;21(20):6198-204. doi: 10.1016/j.bmc.2013.04.082. Epub 2013 May 9.
Many biological functions of microRNA (miRNA) have been identified in the past decade. However, a single miRNA can regulate multiple gene targets, thus it has been a challenge to elucidate the specific functions of each miRNA in different locations and times. New chemical tools make it possible to modulate miRNA activity with higher spatiotemporal resolution. Here, we describe light-activated (caged) constructs for switching let-7 miRNA 'on' or 'off' with 365 nm light in developing zebrafish embryos.
在过去的十年中,人们已经鉴定出 microRNA(miRNA)的许多生物学功能。然而,单个 miRNA 可以调控多个基因靶标,因此阐明每个 miRNA 在不同位置和时间的特定功能一直是一个挑战。新的化学工具使得以更高的时空分辨率调节 miRNA 活性成为可能。在这里,我们描述了用于在发育中的斑马鱼胚胎中用 365nm 光开启或关闭 let-7 miRNA 的光激活(笼状)构建体。