环状笼状吗啉代寡核苷酸：胚胎基因功能的构象门控探针。
Cyclic caged morpholinos: conformationally gated probes of embryonic gene function.
机构信息
Department of Chemical and Systems Biology, Stanford University School of Medicine, CA 94305, USA.
出版信息
Angew Chem Int Ed Engl. 2012 Jul 9;51(28):6908-11. doi: 10.1002/anie.201201690. Epub 2012 Jun 11.
Abstract
Feeling a bit cagey: morpholino-based antisense reagents have been caged through oligonucleotide cyclization, enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.
摘要
感觉有点局促不安
通过寡核苷酸环化使基于 morpholino 的反义试剂被封闭，从而能够在斑马鱼胚胎和幼虫中实现对基因表达的光控。使用这些试剂，研究了发育中的胰腺中外分泌细胞命运决定的时间。
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