Department of Laboratory Animal Center, Liaoning Medical University, Jinzhou 121001, China.
Virus Res. 2013 Sep;176(1-2):78-82. doi: 10.1016/j.virusres.2013.04.018. Epub 2013 May 28.
The genetic diversity of seven northern China isolated Sacbrood virus strains (SBV) has been analyzed, and hypervariable regions of the VP1 gene of 7 SBV were sequenced and characterized, in order to obtain epidemiological and immunological information, and to suggest typing criteria for SBV. Sequence analysis of hypervariable regions of the VP1 gene in the genome of these isolates revealed a sequence homology of 91.0-99.3% among all seven local SBV isolates from Apis cerana from China, with a similarity of 93.3-100.0% in deduced amino acid sequences. These local isolates shared 87.4-92.8% sequence homology with six SBV reference strains in GenBank (including two SBV reference strains from Apis cerana from China), which represents a 91.8-97.6% similarity in deduced amino acid sequences. Genetic analysis also showed that five SBV strains from Apis cerana from China had a 13-amino-acid deletion at amino acid positions 287-299, and two SBV strains infecting the Korean honeybee had a 17-amino-acid deletion at amino acid positions 284-300 in comparison with other SBV. Phylogenetic analysis revealed two major groups (AC genotype SBV infecting Apis cerana and AM genotype SBV infecting Apis mellifera). The AC genotype could be further divided into subgroups. Based on the results of phylogenetic analysis, a similarity scan of SBV nucleotide sequences was carried out by using Simplot software and results in similar results. Our results suggest possible typing criteria for SBV based on the phylogenetic tree and sequence homology, and also that the virus has host specificity and regional variations.
对中国北方 7 株分离自自然发病蜂群的 Sacbrood 病毒（SBV）的遗传多样性进行了分析，对 7 株 SBV 的 VP1 基因高变区进行了测序和特征分析，以期获得流行病学和免疫学信息，并为 SBV 提出分型标准。对这些分离株基因组 VP1 基因高变区的序列分析表明，中国中蜂来源的 7 株地方 SBV 之间的核苷酸序列同源性为 91.0-99.3%，推导的氨基酸序列同源性为 93.3-100.0%。这些地方分离株与 GenBank 中的 6 株 SBV 参考株（包括 2 株来自中国中蜂的 SBV 参考株）的核苷酸序列同源性为 87.4-92.8%，推导的氨基酸序列同源性为 91.8-97.6%。遗传分析还表明，来自中国中蜂的 5 株 SBV 分离株在氨基酸位置 287-299 处有 13 个氨基酸缺失，而感染韩国蜜蜂的 2 株 SBV 分离株在氨基酸位置 284-300 处有 17 个氨基酸缺失，与其他 SBV 相比。系统进化分析显示，SBV 存在 2 个主要基因型（感染中华蜜蜂的 AC 基因型和感染西方蜜蜂的 AM 基因型）。AC 基因型可进一步分为亚群。基于系统进化分析的结果，利用 Simplot 软件对 SBV 核苷酸序列进行相似性扫描，结果相似。我们的研究结果表明，基于系统进化树和序列同源性，SBV 可能存在分型标准，同时也表明该病毒具有宿主特异性和地域变异。
