Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
Virus Res. 2015 Feb 16;198:15-21. doi: 10.1016/j.virusres.2014.12.008. Epub 2014 Dec 16.
Sacbrood virus (SBV), a causative agent of larval death in honeybees, is one of the most devastating diseases in bee industry throughout the world. Lately the Korean Sacbrood virus (KSBV) induced great losses in Korean honeybee (Apis cerana) colonies. However, there is no culture system available for honeybee viruses, including SBV, therefore, the research on honeybee viruses is practically limited until present. In this study, we investigated the growth and replication of SBV in cell cultures. The replication signs of KSBV after passages from mammalian cells was identified and confirmed by using combined approaches with nested, quantitative, negative-strand PCR and electron microscopy along with in vivo experiment. The results revealed that mammalian cell lines, including Vero cells could support the replication KSBV. Although there were no signs of cytopathic effect (CPE) in cells, it was for the first time demonstrated that SBV could be replicated in cells through the sequential passages linked with cell adaptation. KSBV from the present study would be a valuable source to understand the mechanism of pathogenicity of sacbrood virus in the future.
Sacbrood 病毒（SBV）是一种导致蜜蜂幼虫死亡的病原体，是全球蜜蜂产业中最具破坏性的疾病之一。最近，韩国 Sacbrood 病毒（KSBV）导致韩国蜜蜂（Apis cerana）蜂群遭受巨大损失。然而，目前还没有针对蜜蜂病毒（包括 SBV）的培养系统，因此，直到现在，对蜜蜂病毒的研究实际上受到限制。在这项研究中，我们研究了 SBV 在细胞培养中的生长和复制。通过使用巢式、定量、负链 PCR 和电子显微镜以及体内实验相结合的方法，鉴定并确认了 KSBV 在传代后从哺乳动物细胞中复制的迹象。结果表明，包括 Vero 细胞在内的哺乳动物细胞系可以支持 KSBV 的复制。尽管细胞中没有出现细胞病变效应（CPE）的迹象，但这是首次证明 SBV 可以通过与细胞适应相关的连续传代在细胞中复制。本研究中的 KSBV 将成为未来理解 Sacbrood 病毒致病性机制的有价值的资源。
