Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark.
Mol Cell Proteomics. 2013 Sep;12(9):2497-508. doi: 10.1074/mcp.M113.027532. Epub 2013 May 29.
The fungus Septoria tritici causes the disease septoria tritici blotch in wheat, one of the most economically devastating foliar diseases in this crop. To investigate signaling events and defense responses in the wheat-S. tritici interaction, we performed a time-course study of S. tritici infection in resistant and susceptible wheat using quantitative proteomics and phosphoproteomics, with special emphasis on the initial biotrophic phase of interactions. Our study revealed an accumulation of defense and stress-related proteins, suppression of photosynthesis, and changes in sugar metabolism during compatible and incompatible interactions. However, differential regulation of the phosphorylation status of signaling proteins, transcription and translation regulators, and membrane-associated proteins was observed between two interactions. The proteomic data were correlated with a more rapid or stronger accumulation of signal molecules, including calcium, H2O2, NO, and sugars, in the resistant than in the susceptible cultivar in response to the infection. Additionally, 31 proteins and 5 phosphoproteins from the pathogen were identified, including metabolic proteins and signaling proteins such as GTP-binding proteins, 14-3-3 proteins, and calcium-binding proteins. Quantitative PCR analysis showed the expression of fungal signaling genes and genes encoding a superoxide dismutase and cell-wall degrading enzymes. These results indicate roles of signaling, antioxidative stress mechanisms, and nutrient acquisition in facilitating the initial symptomless growth. Taken in its entirety, our dataset suggests interplay between the plant and S. tritici through complex signaling networks and downstream molecular events. Resistance is likely related to several rapidly and intensively triggered signal transduction cascades resulting in a multiple-level activation of transcription and translation processes of defense responses. Our sensitive approaches and model provide a comprehensive (phospho)proteomics resource for studying signaling from the point of view of both host and pathogen during a plant-pathogen interaction.
小麦壳针孢菌引起的小麦叶枯病是这种作物中最具经济破坏性的叶部病害之一。为了研究小麦与壳针孢菌互作中的信号事件和防御反应，我们使用定量蛋白质组学和磷酸化蛋白质组学对感病和抗病小麦进行了壳针孢菌感染的时间过程研究，特别强调互作的初始生物亲和阶段。我们的研究表明，在亲和和非亲和互作中，防御和应激相关蛋白积累，光合作用受到抑制，糖代谢发生变化。然而，在两种互作中，信号蛋白、转录和翻译调节剂以及膜相关蛋白的磷酸化状态的差异调节被观察到。蛋白质组学数据与信号分子（包括钙、H2O2、NO 和糖）在抗病品种中比在感病品种中更快或更强地积累相关，这是对感染的响应。此外，从病原体中鉴定出 31 种蛋白质和 5 种磷酸化蛋白质，包括代谢蛋白和信号蛋白，如 GTP 结合蛋白、14-3-3 蛋白和钙结合蛋白。定量 PCR 分析显示真菌信号基因和编码超氧化物歧化酶和细胞壁降解酶的基因的表达。这些结果表明，信号转导、抗氧化应激机制和养分获取在促进最初无症状生长中起作用。总的来说，我们的数据集表明，植物和 S. tritici 通过复杂的信号网络和下游分子事件相互作用。抗性可能与几个快速和强烈触发的信号转导级联有关，导致防御反应的转录和翻译过程的多层次激活。我们的敏感方法和模型为从宿主和病原体的角度研究植物-病原体互作中的信号提供了一个全面的（磷酸化）蛋白质组学资源。
