Lan Ming-Ying, Hsu Yen-Bin, Hsu Chih-Hung, Ho Ching-Yin, Lin Jin-Ching, Lee Sheng-Wei
Department of Otolaryngology, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.
Auris Nasus Larynx. 2013 Dec;40(6):563-8. doi: 10.1016/j.anl.2013.04.011. Epub 2013 May 27.
Nasopharyngeal carcinoma (NPC) is a rare malignancy in most parts of the world, but is a common cancer in southern Asia. Local recurrent disease and distant metastasis of NPC are still the unsolved problems. Recently, gold nanoparticles (AuNPs) have been developed as potential in vivo diagnostic and therapeutic agents. However, their role on nasopharyngeal cancer remains unknown. The object of this study is to investigate if AuNPs can be used as a new therapeutic agent for NPC by evaluating their anti-tumor effect in vitro.
The AuNPs were prepared by the reduction of chloroauric acid to neutral gold. Their size distribution and microstructures were characterized by transmission electron microscopy (TEM). To evaluate their cytotoxic effect, NPC cell line TW01 and Human Nasal Epithelial Cells (HNEpC) were cultured in various concentrations of AuNPs for 3 days. Cell viability was evaluated by Trypan Blue viability assay while morphologic findings were observed via light microscopy. Terminal deoxynucleotidyltransferase-mediated dUPT nick end labeling (TUNEL) assay was used to detect apoptosis.
AuNPs prepared in this study had an average diameter of 20.5nm and they were observed under light microscopy as dark material aggregated in the cells after treatment. Contrary to the HNEpC, the AuNPs reduced cell viability of NPC cell in a concentration-dependant manner by Trypan Blue assay, especially at high concentration. Besides, cell apoptosis was demonstrated by positive TUNEL assay.
The AuNP possesses specific imaging properties and is cytotoxic to NPC cells at high concentrations.
鼻咽癌（NPC）在世界大部分地区是一种罕见的恶性肿瘤，但在南亚却是一种常见癌症。鼻咽癌的局部复发和远处转移仍然是尚未解决的问题。最近，金纳米颗粒（AuNPs）已被开发为潜在的体内诊断和治疗剂。然而，它们在鼻咽癌上的作用仍然未知。本研究的目的是通过评估其体外抗肿瘤作用来研究AuNPs是否可作为鼻咽癌的一种新的治疗剂。
通过将氯金酸还原为中性金来制备AuNPs。通过透射电子显微镜（TEM）表征其尺寸分布和微观结构。为了评估其细胞毒性作用，将NPC细胞系TW01和人鼻上皮细胞（HNEpC）在不同浓度的AuNPs中培养3天。通过台盼蓝活力测定评估细胞活力，同时通过光学显微镜观察形态学发现。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）测定用于检测细胞凋亡。
本研究中制备的AuNPs平均直径为20.5nm，在光学显微镜下观察到处理后细胞中聚集有深色物质。与HNEpC相反，通过台盼蓝测定，AuNPs以浓度依赖性方式降低NPC细胞的活力，尤其是在高浓度时。此外，TUNEL测定呈阳性证明细胞凋亡。
AuNP具有特定的成像特性，并且在高浓度下对NPC细胞具有细胞毒性。
