金纳米粒子的提取合成及其对鼻咽癌细胞的体外抗癌活性。
Synthesis of gold nanoparticles with extract and their in vitro anticancer potential on nasopharyngeal carcinoma cells.
机构信息
Department of Otolaryngology - Head and Neck Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China,
Department of Otolaryngology - Head and Neck Surgery, Weihai Municipal Hospital, Weihai, Shandong 264200, China.
出版信息
Int J Nanomedicine. 2018 Nov 1;13:7047-7059. doi: 10.2147/IJN.S180138. eCollection 2018.
BACKGROUND
Nasopharyngeal cancer (NPC) is one of the subtypes of head and neck cancers. It occurs rarely, and its prevalence depends mainly on geographical location. Modern-day research is focused on coupling nanotechnology and traditional medicine for combating cancers. Gold nanoparticles (AuNPs) were synthesized from (Sx) leaf extract using reduction method.
METHODS
Characterization of the synthesized AuNPs was done by different techniques such as ultraviolet-visible spectrum absorption, X-ray diffraction, dynamic light scattering, Fourier transform infrared spectroscopy, transmission electron microscopy, and energy-dispersive X-ray analysis.
RESULTS
All the results showed the successful green synthesis of AuNPs from Sx, which induced apoptosis of C666-1 cell line (NPC cell line). There was a decline in both cell viability and colony formation in C666-1 cells upon treatment with Sx-AuNPs. The cell death was proved to be caused by autophagy and mitochondrial-dependent apoptotic pathway.
CONCLUSION
Thus, due to their anticancer potential, these nanoparticles coupled with Sx can be used for in vivo applications and clinical research in future.
背景
鼻咽癌（NPC）是头颈部癌症的一种亚型。它发病率较低，其流行程度主要取决于地理位置。目前的研究集中在将纳米技术与传统医学相结合来治疗癌症。本研究采用还原法从（Sx）叶提取物中合成了金纳米粒子（AuNPs）。
方法
采用紫外可见光谱吸收、X 射线衍射、动态光散射、傅里叶变换红外光谱、透射电子显微镜和能谱分析等不同技术对合成的 AuNPs 进行了表征。
结果
所有结果均表明，成功地从 Sx 中绿色合成了 AuNPs，AuNPs 可诱导 C666-1 细胞系（NPC 细胞系）凋亡。Sx-AuNPs 处理后 C666-1 细胞的细胞活力和集落形成均下降。细胞死亡被证明是由自噬和线粒体依赖性凋亡途径引起的。
结论
因此，由于其抗癌潜力，这些与 Sx 结合的纳米粒子可用于未来的体内应用和临床研究。
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