Inazu T, Taniguchi T, Yanagi S, Yamamura H
Department of Biochemistry, Fukui Medical School, Japan.
Biochem Biophys Res Commun. 1990 Jul 16;170(1):259-63. doi: 10.1016/0006-291x(90)91268-w.
The ability of vanadate with H2O2 to stimulate protein-tyrosine phosphorylation and aggregation in intact human platelets was observed. Upon stimulation by these agents, there was a change in the amount of protein-tyrosine phosphorylation of four bands with molecular masses of 80-, 76-, 53- and 38-kDa proteins. The tyrosine phosphorylation in these four bands increased gradually and reached a maximum level at 2 min and then decreased. Aggregation of platelets was also induced by these agents in a dose dependent manner. The observation that the aggregation was preceded by the increase in tyrosine phosphorylation of these proteins suggests that tyrosine phosphorylation may be involved in an early stage of platelet aggregation.
观察到钒酸盐与过氧化氢能够刺激完整人血小板中的蛋白质酪氨酸磷酸化和聚集。在这些试剂的刺激下，分子量分别为80 kDa、76 kDa、53 kDa和38 kDa的四种蛋白质条带的蛋白质酪氨酸磷酸化量发生了变化。这四条带中的酪氨酸磷酸化逐渐增加，在2分钟时达到最高水平，然后下降。这些试剂还以剂量依赖性方式诱导血小板聚集。这些蛋白质酪氨酸磷酸化增加先于聚集的观察结果表明，酪氨酸磷酸化可能参与血小板聚集的早期阶段。
