Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, Egypt.
J Fluoresc. 2013 Sep;23(5):1077-87. doi: 10.1007/s10895-013-1237-3. Epub 2013 May 31.
A highly sensitive, simple and rapid spectrofluorimetric method was developed for the determination of Terbinafine HCl (TRH) and linezolid (LNZ) in their pharmaceutical formulations. The proposed method is based on measuring the native fluorescence of the studied drugs in water at 336 nm after excitation at 275 nm for TRH and 375 nm after excitation at 254 nm for LNZ. The fluorescence-concentration plots were rectilinear over the range of 0.02-0.15 μg/mL for TRH and 0.5-5.0 μg/mL for LNZ. With lower detection limits of 3.0 and 110.0 ng/mL and a lower quantification limit of 9.0 and 320.0 ng/mL for TRH and LNZ, respectively. The method was successfully applied to the analysis of TRH in its commercial tablets, cream, gel and spray formulations and the results were in good agreement with those obtained with the official method. In addition the method was also applied to the analysis of LNZ in its capsule and I.V solution and the results were in good agreement with those obtained with the comparison method. The effect of sensitizers was studied. The method was extended to the determination of the studied drugs in spiked human plasma and the results were satisfactory.
建立了一种测定盐酸特比萘芬（TRH）和利奈唑胺（LNZ）的高灵敏度、简单、快速的荧光分光光度法。该方法基于在水相中测量研究药物的固有荧光，TRH 在 275nm 激发下于 336nm 处测量，LNZ 在 254nm 激发下于 375nm 处测量。对于 TRH，荧光浓度图在 0.02-0.15μg/mL 范围内呈线性，对于 LNZ，在 0.5-5.0μg/mL 范围内呈线性。对于 TRH 和 LNZ，检测限分别为 3.0 和 110.0ng/mL，定量限分别为 9.0 和 320.0ng/mL。该方法成功应用于 TRH 商业片剂、乳膏、凝胶和喷雾剂的分析，结果与官方方法一致。此外，该方法还应用于 LNZ 胶囊和静脉注射溶液的分析，结果与比较法一致。研究了增敏剂的影响。该方法扩展到测定人血浆中加标研究药物的含量，结果令人满意。
