Etcheverría A I, Arroyo G H, Alzola R, Parma A E
Laboratorio de Inmunoquímica y Biotecnología, Departamento de Sanidad Animal y Medicina Preventiva, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro Provincia de Buenos Aires, Pinto 399 (7000), Tandil, Argentina.
ISRN Microbiol. 2011 Dec 11;2011:697020. doi: 10.5402/2011/697020. Print 2011.
Enterohemorrhagic E. coli strains (EHEC) had emerged as foodborne pathogens and cause in human diarrhea and hemolytic-uremic syndrome. Because of the widespread distribution of EHEC serotypes and O157 and non-O157 in cattle population, its control will require interventions at the farm level such as the administration of probiotics that produce inhibitory metabolites. E. coli O157:H7 shows tissue tropisms for the gastrointestinal tract (GIT) of cattle. The aim of this study was to test the ability of a colicinogenic E. coli (isolated from bovine) to reduce the adherence of E. coli O157:H7 to HEp-2 cells and to GIT of cattle. We inoculated HEp-2 cells and bovine colon explants with both kinds of strains. Colicinogenic E. coli was able to reduce the adherence of E. coli O157:H7 to HEp-2 cells and to bovine tissues.
肠出血性大肠杆菌菌株（EHEC）已成为食源性病原体，可导致人类腹泻和溶血尿毒综合征。由于EHEC血清型以及O157和非O157在牛群中广泛分布，对其进行控制需要在农场层面采取干预措施，例如施用能产生抑制性代谢物的益生菌。大肠杆菌O157:H7对牛的胃肠道（GIT）表现出组织嗜性。本研究的目的是测试一种产大肠杆菌素的大肠杆菌（从牛分离得到）降低大肠杆菌O157:H7对HEp-2细胞以及牛胃肠道黏附的能力。我们用这两种菌株接种HEp-2细胞和牛结肠外植体。产大肠杆菌素的大肠杆菌能够降低大肠杆菌O157:H7对HEp-2细胞和牛组织的黏附。
