Blibech Monia, Ellouz Ghorbel Raoudha, Chaari Fatma, Dammak Ilyes, Bhiri Fatma, Neifar Mohamed, Ellouz Chaabouni Semia
Unité Enzymes et Bioconversion, Ecole National d'ingénieurs de Sfax, Université de Sfax, Route de Soukra 3038, Sfax, Tunisia.
ISRN Microbiol. 2011 Oct 24;2011:938347. doi: 10.5402/2011/938347. Print 2011.
By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.
通过采用补料分批策略，与在相思树种子上进行分批培养相比，奥克西坦青霉甘露聚糖酶的产量几乎可以翻倍（分别为76 U/mL和41 U/mL）。此外，从摇瓶发酵到补料分批发酵，酶活性提高了10倍。这些产量水平比在椰糠上获得的产量高3倍。使用相思树种子粉作为诱导底物的高甘露聚糖酶产量表明该培养过程适用于工业规模开发。
