Romero-Pastrana Francisco
FRP Genética, 115A Ote 1417-1, 72520 Puebla, PUE, Mexico.
ISRN Microbiol. 2012 Mar 1;2012:186915. doi: 10.5402/2012/186915. Print 2012.
The primary underlying cause of cervical cancer is infection with one or more high-risk (HR) types of the human papilloma virus (HPV). Detection and typing of HPV have been commonly carried out by PCR-based assays, where HPV detection and typing are two separate procedures. Here, we present a multiplex PCR-based HPV typing assay that detects 20 HPV types (15 HR, 3 probably HR and 2 low risk) using type-specific primers and agarose gel electrophoresis. 46 cervical, urethral, and biopsy samples were analyzed by both Multiplex PCR and PGMY09/11 consensus PCR, and results were compared. 611 samples were further analyzed by Multiplex PCR, 282 were positive for HR HPV, and 101 showed multiple HR HPV infections. The relatively ease and economic accessibility of the method and its improved ability to detect high-risk HPV types in multiple HPV-infected samples make it an attractive option for HPV testing.
宫颈癌的主要潜在病因是感染一种或多种高危（HR）型人乳头瘤病毒（HPV）。HPV的检测和分型通常通过基于聚合酶链反应（PCR）的检测方法进行，其中HPV检测和分型是两个独立的程序。在此，我们展示了一种基于多重PCR的HPV分型检测方法，该方法使用型特异性引物和琼脂糖凝胶电泳检测20种HPV类型（15种高危型、3种可能的高危型和2种低危型）。通过多重PCR和PGMY09/11共识PCR对46份宫颈、尿道和活检样本进行分析，并比较结果。通过多重PCR对611份样本进行进一步分析，282份高危型HPV呈阳性，101份显示多重高危型HPV感染。该方法相对简便且经济可行，并且在多个HPV感染样本中检测高危型HPV类型的能力有所提高，使其成为HPV检测的一个有吸引力的选择。
