Liu Lin, Li Xiu-Le, Gao Jian-Ping, Kong Ying-Jun, Wang Ming-Lin, Zhang Gui-Feng, Su Zhi-Guo
College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
Zhongguo Zhong Yao Za Zhi. 2013 Mar;38(5):714-9.
Edible bird's nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird's nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird's nest. More than 20 species of peptides in the digested mixture were identified. The sequences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic digestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird's nest.
燕窝含有大量糖蛋白。糖蛋白的糖基化不均一性常常导致分子量范围广泛，以及蛋白质分离和表征的困难。本文采用多次提取法提取燕窝中的蛋白质，然后用PNgase F和胰蛋白酶进行消化。消化混合物用高效液相色谱法分离，并根据串联质谱数据搜索鉴定肽段。结果表明，提取的蛋白质占燕窝总蛋白的79.7%。消化混合物中鉴定出20多种肽段。这些肽段的序列与Swiss-prot中的一些蛋白质显示出相似性。该研究表明，去糖基化、胰蛋白酶消化结合高效液相色谱-串联质谱是表征燕窝中蛋白质的合适策略。
