Guan Yan, Li Chun, Shi Jing-Jin, Zhou Hua-Na, Liu Li, Wang Yan, Pu Yan-Ping
Anhui University of Traditional Chinese Medicine, Hefei 230038, China.
Zhongguo Zhong Yao Za Zhi. 2013 Mar;38(5):731-5.
To observe the effect of the combination of sub-MIC sodium houttuyfonate and erythromycin on biofilm of Staphylococcus epidermidis.
The serial dilution method was adopted to determine MIC of the combination of sodium houttuyfonate and erythromycin on S. epidermidis; the checkerboard method was used to evaluate the combination of sodium houttuyfonate and erythromycin on suspended bacteria of S. epidermidis; S. epidermidis biofilm was built in vitro, and XTT reduction assay was used to evaluate the effect of the combination of sub-MIC sodium houttuyfonate and erythromycin on the adhesion of S. epidermidis and bacterial metabolism inside the biofilm. Microscope was applied to observe the impact the single administration and combination of the two medicines under sub-MIC on biofilm morphology of S. epidermidis.
The MIC of sodium houttuyfonate and erythromycin were 62.5, 7.812 5 mg x L(-1), respectively. The combination of 1/8MIC sodium houttuyfonate and 1/2MIC erythronmycin showed a synergistic effect on S. epidermidis. Sodium houttuyfonate, erythromycin and their combination had an inhibitory effect on the adhesion and metabolism of S. epidermidis biofilm bacteria, and made impact on the morphology of S. epidermidis biofilm.
The sub-MIC sodium houttuyfonate and erythromycin have an inhibitory effect on S. epidermidis biofilm. The combination of sodium houttuyfonate and erythromycin shows a synergistic effect in inhibiting suspended bacteria and biofilm of S. epidermidis, particularly in inhibiting the metabolism of S. epidermidis biofilm bacteria and impacting the morphology of biofilm.
观察亚抑菌浓度的鱼腥草素钠与红霉素联合应用对表皮葡萄球菌生物膜的影响。
采用系列稀释法测定鱼腥草素钠与红霉素联合应用对表皮葡萄球菌的最低抑菌浓度（MIC）；采用棋盘法评价鱼腥草素钠与红霉素联合应用对表皮葡萄球菌浮游菌的作用；体外构建表皮葡萄球菌生物膜，采用XTT还原法评价亚抑菌浓度的鱼腥草素钠与红霉素联合应用对表皮葡萄球菌生物膜黏附及生物膜内细菌代谢的影响。应用显微镜观察亚抑菌浓度下两种药物单独及联合应用对表皮葡萄球菌生物膜形态的影响。
鱼腥草素钠和红霉素的MIC分别为62.5、7.812 5 mg·L-1。1/8MIC鱼腥草素钠与1/2MIC红霉素联合应用对表皮葡萄球菌表现为协同作用。鱼腥草素钠、红霉素及其联合应用对表皮葡萄球菌生物膜细菌的黏附及代谢均有抑制作用，并对表皮葡萄球菌生物膜形态产生影响。
亚抑菌浓度的鱼腥草素钠和红霉素对表皮葡萄球菌生物膜有抑制作用。鱼腥草素钠与红霉素联合应用在抑制表皮葡萄球菌浮游菌及生物膜方面表现为协同作用，尤其在抑制表皮葡萄球菌生物膜细菌代谢及影响生物膜形态方面。
