Cheng Hui-Juan, Yang Min, Wang Chang-Zhong, Zhang Chang-Feng, Chu Chun-Yun
Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(2):296-9.
To investigate the resistant effect of houttuyfonate sodium (SH) combined with imipenem (IMP) against Pseudomonas aeruginosa (Pa) biofilms.
The two-fold dilution method was used to examine the minimum inhibitory concentration (MIC) of the tested drug. The crystal violet staining was applied to detect the effect of the combination of 1/2MIC, 1MIC, 2MIC of SH, single IMP, 1/2MIC of SH and IMP of various concentrations on the clearance rate of adherent bacteria, growth of biofilms and alginate production. Fluorescein diacetate (FDA)-propidium iodide (PI) doubling staining assay was employed to observe the bacterial viability and morphological changes after membrane dispersion of each drug group.
Sodium houttuyfonate could enhance the effect of IMP against pseudomonas aeruginosa biofilms. Particularly, the combination group with the concentration of 2MIC showed the highest effect, with P < 0.001 compared with the negative control group. The above results were proved by the bacterial viability and biofilm morphology under fluorescence microscope.
After being combined with imipenem, sodium houttuyfonate shows a higher effect against biofilms. It is expected that the combination of the two drugs could improve the clinical efficacy of associated infections.
探讨鱼腥草素钠（SH）联合亚胺培南（IMP）对铜绿假单胞菌（Pa）生物膜的耐药作用。
采用二倍稀释法检测受试药物的最低抑菌浓度（MIC）。应用结晶紫染色检测1/2MIC、1MIC、2MIC的SH、单一IMP、1/2MIC的SH与不同浓度IMP联合作用对黏附菌清除率、生物膜生长及藻酸盐产生的影响。采用荧光素二乙酸酯（FDA）-碘化丙啶（PI）双重染色法观察各药物组膜分散后细菌活力及形态变化。
鱼腥草素钠可增强IMP对铜绿假单胞菌生物膜的作用。特别是2MIC浓度的联合组效果最佳，与阴性对照组相比，P<0.001。荧光显微镜下的细菌活力和生物膜形态证实了上述结果。
鱼腥草素钠与亚胺培南联合后对生物膜有更高的作用效果。预计两种药物联合可提高相关感染的临床疗效。
