TPX2 基因的上调与食管鳞癌恶性程度的增强有关。
Upregulation of the TPX2 gene is associated with enhanced tumor malignance of esophageal squamous cell carcinoma.
机构信息
Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
出版信息
Biomed Pharmacother. 2013 Oct;67(8):751-5. doi: 10.1016/j.biopha.2013.04.004. Epub 2013 May 14.
PURPOSE
To explore the expression of TPX2 and its significance in esophageal squamous cell carcinoma (ESCC) tissue and approach relationship between the TPX2 and clinicopathological characteristic of esophageal squamous cell carcinoma.
METHOD
RT-PCR and immunohistochemical staining were used to compare the expression of TPX2 in 62 esophageal squamous cell carcinoma, 31 atypical hyperplasia and 62 normal esophageal mucosa.
RESULTS
In ESCC, atypical hyperplasia and in normal mucous membrane tissues, the positive rate of TPX2 protein expression was 85.5% (53/62), 51.6% (16/31) and 4.8% (3/62); the positive rate of TPX2 mRNA expression was 65.5% (40/62), 35.5 (11/31) and 4.83% (3/62). The expression of TPX2 protein and mRNA were correlated with invasive depth and lymphatic metastasis of ESCC (P<0.01).
CONCLUSIONS
Overexpression of TPX2 may be risk factor of lymph node in esophageal carcinoma, and maybe a potential biomarker for early diagnosis and prognosis of esophageal squamous cell carcinoma.
目的
探讨 TPX2 在食管鳞癌（ESCC）组织中的表达及其意义，并探讨 TPX2 与食管鳞癌临床病理特征的关系。
方法
采用 RT-PCR 和免疫组织化学染色法比较 62 例食管鳞癌、31 例不典型增生和 62 例正常食管黏膜组织中 TPX2 的表达。
结果
在 ESCC、不典型增生和正常黏膜组织中，TPX2 蛋白表达的阳性率分别为 85.5%（53/62）、51.6%（16/31）和 4.8%（3/62）；TPX2mRNA 的阳性率分别为 65.5%（40/62）、35.5%（11/31）和 4.83%（3/62）。TPX2 蛋白和 mRNA 的表达与 ESCC 的浸润深度和淋巴转移相关（P<0.01）。
结论
TPX2 的过表达可能是食管癌淋巴结转移的危险因素，可能是食管鳞癌早期诊断和预后的潜在标志物。
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