西藏糙苏的抗焦虑活性。

Anti-anxiety activity of Stachys tibetica Vatke.

机构信息

Department of Pharmaceutical Sciences, University of Kashmir, Srinagar, India.

出版信息

Chin J Nat Med. 2013 May;11(3):240-4. doi: 10.1016/S1875-5364(13)60022-9.

DOI:10.1016/S1875-5364(13)60022-9

PMID:23725835

Abstract

AIM

S. tibetica Vatke is a herb distributed in the tropical and subtropical regions of the world, including Tibet, China, and India. In India it is found in the cold desert regions of Kargil, Ladakh Valley, and in the mountains of Himachal Pradesh. The traditional practitioners in the Kargil and Ladakh use the natural medicine Stachys tibetica for the treatment of various mental disorders and phobias. The present study is aimed at evaluating the anxiolytic effects of the methanolic extract of the root, stem, leaf, and whole plant material of Stachys tibetica Vatke in rats.

METHODS

Powdered materials (1 kg) of each plant part were subjected to extraction in a Soxhlet apparatus with methanol (95%); to yield 12.8%, 8.3%, 17.2%, and 19.6% W/W extractives, respectively. Extracts were evaluated for their anxiolytic effects using the elevated plus maze (EPM) test in rats.

RESULTS

In the present study, it was found that the methanolic extracts (200 and 400 mg·kg(-1)) of the root, stem, leaf and whole plant of Stachys tibetica Vatke and diazepam (DZ) increased the time spent and the number of entries in the open arm significantly (**P < 0.01), while they decreased the time spent and the number of entries in the closed arm. At the same time, all of the extracts and DZ decreased the time spent at the center of the maze (latency), along with closed arm returns. The head dip counts increased significantly in the rats treated with DZ, SMR400, SML400 and SMW400 in the open arm of EPM, which was a sign of reduction anxiety. The DZ and SMW did not show the fecal bolus, while other groups had reduced fecal bolus (**P < 0.01) as compared to control. These allied parameters helped to assess the anxiolytic potential of Stachys tibetica Vatke. Whole plant and leaf materials have shown the maximum activity, the root intermediate while the stem had the least anxiolytic activity (*P < 0.05, **P < 0.01) in EPM.

CONCLUSION

The results strongly justify the use of this plant for the treatment of anxiety. Further studies are in progress in this laboratory to isolate and identify the components responsible for the anxiolytic activity and the mechanism of action involved.

摘要

目的

西藏香青（S. tibetica Vatke）是一种分布于世界热带和亚热带地区的草本植物，包括中国西藏和印度。在印度，它生长在克什米尔的寒冷沙漠地区、拉达克山谷和喜马偕尔邦的山区。卡吉尔和拉达克的传统从业者使用天然药物西藏香青治疗各种精神障碍和恐惧症。本研究旨在评估西藏香青根、茎、叶和全植物甲醇提取物在大鼠中的抗焦虑作用。

方法

将各植物部位（1 公斤）的粉末置于索氏提取器中用甲醇（95%）提取；分别得到 12.8%、8.3%、17.2%和 19.6%（W/W）提取物。采用高架十字迷宫（EPM）试验评估提取物的抗焦虑作用。

结果

本研究发现，西藏香青根、茎、叶和全植物的甲醇提取物（200 和 400mg·kg(-1))和地西泮（DZ）增加了大鼠在开放臂中的停留时间和进入次数（**P < 0.01），同时减少了在封闭臂中的停留时间和进入次数。同时，所有提取物和 DZ 都减少了在迷宫中心的停留时间（潜伏期），同时减少了对封闭臂的返回。DZ、SMR400、SML400 和 SMW400 处理的大鼠在 EPM 的开放臂中出现明显的头部浸入次数增加，这是焦虑减轻的标志。DZ 和 SMW 没有显示粪便球，而其他组的粪便球减少（**P < 0.01）与对照组相比。这些相关参数有助于评估西藏香青 Vatke 的抗焦虑潜力。全植物和叶材料表现出最大的活性，根材料表现为中等活性，而茎材料表现出最小的抗焦虑活性（*P < 0.05，**P < 0.01）在 EPM 中。