Institute of Pharmacology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Phytother Res. 2010 Sep;24(9):1309-16. doi: 10.1002/ptr.3106.
We performed a basic behavioral characterization of methanol extracts of four Balkan endemic Stachys taxa: S. anisochila (SA), S. beckeana (SB), S. plumosa (SP) and S. alpina subsp. dinarica (SAD). The behavioral activity of extracts dosed intraperitoneally in the range 100-400 mg/kg was examined in adult male Wistar rats, in the elevated plus maze, spontaneous locomotor activity, and grip strength tests, mainly predictive of anxiolytic, sedative and myorelaxant actions, respectively. All investigated Stachys extracts lacked anxiolytic or myorelaxant activities, while SB at 400 mg/kg exerted an anxiogenic-like effect. The study with the selective antagonist beta-CCt showed that the sedative effect of SAD was only partially mediated by GABAA receptors containing the alpha1-subunit. While discernible, the behavioral effects of SA and SP were not distinct. In all extracts, chlorogenic acid and verbascoside were identified. In SA, SB, and SAD the flavonoid fraction was constituted of isoscutellarein and hypolaetine glycosides, while in SP chrysoeriol and apigenin glycosides were present. The results reveal the psychotropic potential of four endemic Stachys taxa, of which SAD appeared most promising as a natural sedative.
我们对四个巴尔干地方性 Stachys 分类群的甲醇提取物进行了基本的行为特征描述：S. anisochila (SA)、S. beckeana (SB)、S. plumosa (SP) 和 S. alpina subsp. dinarica (SAD)。在成年雄性 Wistar 大鼠中，以 100-400mg/kg 的剂量腹腔内给药，研究了提取物的行为活性，在高架十字迷宫、自发运动活性和握力测试中，主要预测抗焦虑、镇静和肌松作用，分别。所有研究的 Stachys 提取物均缺乏抗焦虑或肌松活性，而 400mg/kg 的 SB 则表现出类似焦虑的作用。使用选择性拮抗剂 beta-CCt 的研究表明，SAD 的镇静作用仅部分由含有 alpha1 亚基的 GABA A 受体介导。虽然可以察觉，但 SA 和 SP 的行为作用并不明显。在所有提取物中，均鉴定出绿原酸和毛蕊花糖苷。在 SA、SB 和 SAD 中，类黄酮部分由异鼠李素和 Hypolaetine 糖苷组成，而在 SP 中则存在 Chrysoeriol 和芹菜素糖苷。结果揭示了四个地方性 Stachys 分类群的精神活性潜力，其中 SAD 似乎最有希望成为天然镇静剂。
