Materials Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Anal Chim Acta. 2013 Jun 14;783:65-73. doi: 10.1016/j.aca.2013.04.037. Epub 2013 Apr 29.
Unmodified cysteamine capped nanocrystalline cadmium sulfide quantum dots (Cys-CdS QDs) were demonstrated as a selective turn-on fluorescence sensor for sensing adenosine-5'-triphosphate (ATP) in aqueous solution for the first time. The fluorescence intensity of the Cys-CdS QDs was significantly enhanced in the presence of ATP. In addition, the fluorescence intensity of the Cys-CdS QDs increased when increasing ATP concentrations. On the other hand, other phosphate metabolites and other tested common anions did not significantly alter the fluorescence intensity of the Cys-CdS QDs. In addition, this sensor showed excellent discrimination of pyrophosphate (PPi) from ATP detection. The proposed sensor could efficiently be used for ATP sensing at very low concentration with LOD of 17 μM with the linear working concentration range of 20-80 μM. The feasibility of the proposed sensor for determining ATP in urine samples was also studied, and satisfactory results were obtained.
未修饰的半胱胺封端的纳米晶硫化镉量子点（Cys-CdS QDs）首次被证明是一种在水溶液中用于检测三磷酸腺苷（ATP）的选择性荧光开启传感器。在存在 ATP 的情况下，Cys-CdS QDs 的荧光强度显著增强。此外，随着 ATP 浓度的增加，Cys-CdS QDs 的荧光强度也增加。另一方面，其他磷酸盐代谢物和其他测试的常见阴离子并没有显著改变 Cys-CdS QDs 的荧光强度。此外，该传感器对检测焦磷酸盐（PPi）与 ATP 的检测具有出色的区分能力。该传感器可用于在非常低的浓度下检测 ATP，检测限为 17 μM，线性工作浓度范围为 20-80 μM。还研究了该传感器用于测定尿液样品中 ATP 的可行性，获得了令人满意的结果。
