中国仓鼠卵巢细胞的离子传输和细胞质电导率的定量模型。
Quantitative Model for Ion Transport and Cytoplasm Conductivity of Chinese Hamster Ovary Cells.
机构信息
Department of Electrical and Computer Engineering, University of Manitoba, Winnipeg, R3T 2N2, Canada.
Department of Microbiology, University of Manitoba, Winnipeg, R3T 2N2, Canada.
出版信息
Sci Rep. 2018 Dec 13;8(1):17818. doi: 10.1038/s41598-018-36127-3.
In mammalian cells cytoplasm ion concentrations and hence cytoplasm conductivity is an important indicator of their physiological state. Changes in the cytoplasm conductivity has been associated with physiological changes such as progression of cancer and apoptosis. In this work, a model that predicts the effects of physiological changes in ion transport on the cytoplasm conductivity of Chinese hamster ovary (CHO) cells is demonstrated. We determined CHO-specific model parameters, Na/K ATPase pumps and ion channels densities, using a flux assay approach. The obtained sodium (P), potassium (P) and chloride (P) permeability and Na/K ATPase pump density were estimated to be 5.6 × 10 cm/s, 5.6 × 10 cm/s, 3.2 × 10 cm/s and 2.56 × 10 mol/cm, respectively. The model was tested by comparing the model predictions with the experimentally determined temporal changes in the cytoplasm conductivity of Na/K ATPase pump inhibited CHO cells. Cells' Na/K ATPase pumps were inhibited using 5 mM Ouabain and the temporal behavior of their cytoplasm conductivity was measured using dielectrophoresis cytometry. The measured results are in close agreement with the model-calculated values. This model will provide insight on the effects of processes such as apoptosis or external media ion concentration on the cytoplasm conductivity of mammalian cells.
在哺乳动物细胞中，细胞质离子浓度及其电导率是其生理状态的重要指标。细胞质电导率的变化与生理变化有关，如癌症的进展和细胞凋亡。在这项工作中，展示了一种预测离子传输的生理变化对中国仓鼠卵巢（CHO）细胞细胞质电导率影响的模型。我们使用通量测定法确定了 CHO 特异性模型参数，即 Na/K ATPase 泵和离子通道的密度。获得的钠（P）、钾（P）和氯（P）通透性和 Na/K ATPase 泵密度估计分别为 5.6×10−6cm/s、5.6×10−6cm/s、3.2×10−6cm/s 和 2.56×10−6mol/cm。通过将模型预测与 Na/K ATPase 泵抑制的 CHO 细胞的细胞质电导率的实验测定的时间变化进行比较来测试模型。使用 5 mM 哇巴因抑制细胞的 Na/K ATPase 泵，并使用介电泳细胞术测量其细胞质电导率的时间行为。测量结果与模型计算值非常吻合。该模型将为细胞凋亡或外部介质离子浓度等过程对哺乳动物细胞细胞质电导率的影响提供深入了解。
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