Solenov Evgeniy I, Baturina Galina S, Katkova Liubov E, Yang Baoxue, Zarogiannis Sotirios G
Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia.
Novosibirsk State Technical University, Novosibirsk, Russia.
Adv Exp Med Biol. 2023;1398:343-361. doi: 10.1007/978-981-19-7415-1_24.
Water permeability is a key feature of the cell plasma membranes, and it has seminal importance for several cell functions such as cell volume regulation, cell proliferation, cell migration, and angiogenesis to name a few. The transport of water occurs mainly through plasma membrane water channels, aquaporins. Aquaporins have very important function in physiological and pathophysiological states. Due to the above, the experimental assessment of the water permeability of cells and tissues is necessary. The development of new methodologies of measuring water permeability is a vibrant scientific field that constantly develops during the last three decades along with the advances in imaging mainly. In this chapter we describe and critically assess several methods that have been developed for the measurement of water permeability both in living cells and in tissues with a focus in the first category.
水渗透性是细胞质膜的一个关键特性，对多种细胞功能具有至关重要的意义，比如细胞体积调节、细胞增殖、细胞迁移以及血管生成等等。水的运输主要通过质膜水通道，即水孔蛋白来进行。水孔蛋白在生理和病理生理状态下都具有非常重要的功能。基于上述原因，对细胞和组织的水渗透性进行实验评估是很有必要的。测量水渗透性新方法的开发是一个活跃的科学领域，在过去三十年里，主要随着成像技术的进步而不断发展。在本章中，我们将描述并批判性地评估已开发出的几种用于测量活细胞和组织中水渗透性的方法，重点关注第一类方法。
