血浆氨基酸浓度变化对红细胞氨基酸含量的影响。

The effects of changes in plasma amino acid concentrations on erythrocyte amino acid content.

作者信息

Schaefer A, Piquard F, Haberey P

机构信息

Groupe de Recherche en Nutrition Foetale, Université Louis Pasteur, Faculté de Médecine, Strasbourg, France.

出版信息

Clin Biochem. 1990 Jun;23(3):237-40. doi: 10.1016/0009-9120(90)90679-o.

DOI:10.1016/0009-9120(90)90679-o

PMID:2372938

Abstract

In order to determine the effects of large variations in plasma amino acid concentrations upon human erythrocyte amino acid content, the plasma concentration of blood samples was enhanced (x 3.8) by adding amino acids or decreased (x 0.49) by plasma dilution. Before and after incubation (30 s at 37 degrees C), the erythrocyte contents were calculated from whole blood and plasma amino acid concentrations. Large and rapid plasma concentration variations led to significant erythrocyte changes in 11 amino acids. THR, CIT, alpha AB, VAL, MET, ILE, LEU, TYR, PHE, TRP, and ARG. Relationships between erythrocyte and plasma concentrations were determined for these amino acids. These observations were examined in the light of the role played by erythrocytes in blood amino acid transport.

摘要

为了确定血浆氨基酸浓度的大幅变化对人体红细胞氨基酸含量的影响，通过添加氨基酸使血样的血浆浓度提高（3.8倍），或通过血浆稀释使其降低（0.49倍）。在孵育前后（37℃下30秒），根据全血和血浆氨基酸浓度计算红细胞含量。血浆浓度的大幅快速变化导致11种氨基酸的红细胞含量发生显著变化，这些氨基酸分别是苏氨酸（THR）、瓜氨酸（CIT）、α-氨基丁酸（alpha AB）、缬氨酸（VAL）、蛋氨酸（MET）、异亮氨酸（ILE）、亮氨酸（LEU）、酪氨酸（TYR）、苯丙氨酸（PHE）、色氨酸（TRP）和精氨酸（ARG）。确定了这些氨基酸在红细胞和血浆浓度之间的关系。根据红细胞在血液氨基酸运输中所起的作用对这些观察结果进行了研究。