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Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures.从泡盛曲霉液体培养物中纯化和部分表征一种外聚半乳糖醛酸酶。
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2
Optimization of enzymatic clarification of sapodilla juice: a statistical perspective.人心果果汁酶法澄清的优化：统计学视角
Appl Biochem Biotechnol. 2008 Dec;151(2-3):353-63. doi: 10.1007/s12010-008-8198-z. Epub 2008 Aug 1.
3
Production of thermostable pectinase and xylanase for their potential application in bleaching of kraft pulp.生产热稳定果胶酶和木聚糖酶及其在硫酸盐浆漂白中的潜在应用。
J Ind Microbiol Biotechnol. 2007 Dec;34(12):763-70. doi: 10.1007/s10295-007-0251-3. Epub 2007 Aug 28.
4
Purification, characterization, kinetic properties, and thermal behavior of extracellular polygalacturonase produced by filamentous fungus Tetracoccosporium sp.丝状真菌四角孢属（Tetracoccosporium sp.）产生的胞外聚半乳糖醛酸酶的纯化、表征、动力学性质及热行为
Appl Biochem Biotechnol. 2006 Dec;135(3):193-208. doi: 10.1385/abab:135:3:193.
5
Isolation and properties of pectinases from the fungus Aspergillus japonicus.日本曲霉果胶酶的分离与特性
Biochemistry (Mosc). 2003 May;68(5):559-69. doi: 10.1023/a:1023959727067.
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Purification and general properties of pectin methyl esterase from Curvularia inaequalis NRRL 13884 in solid state culture using orange peels as an inducer.以橙皮为诱导剂，在固态培养条件下，对不等弯孢霉NRRL 13884中果胶甲基酯酶的纯化及一般性质研究
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Endo-xylogalacturonan hydrolase, a novel pectinolytic enzyme.内切木糖半乳糖醛酸水解酶，一种新型果胶分解酶。
Appl Environ Microbiol. 2000 Jan;66(1):36-41. doi: 10.1128/AEM.66.1.36-41.2000.
9
Heat-resistant fungi of importance to the food and beverage industry.对食品和饮料行业具有重要意义的耐热真菌。
Crit Rev Microbiol. 1994;20(4):243-63. doi: 10.3109/10408419409113558.
10
Variation in polygalacturonase production among Aspergillus flavus isolates.
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从土壤分离株层出镰刀菌 NFCCI 1769 中生产和纯化外聚半乳糖醛酸酶及其应用。

Production, Purification of Exo-Polygalacturonase from Soil Isolate Paecilomyces variotii NFCCI 1769 and Its Application.

机构信息

Department of Microbiology, SSVPS's Lk. Dr. P. R. Ghogrey Science College, Dhule, 424005 Maharashtra India.

出版信息

Indian J Microbiol. 2012 Jun;52(2):240-6. doi: 10.1007/s12088-011-0162-x. Epub 2011 Feb 14.

DOI:10.1007/s12088-011-0162-x
PMID:23729888
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3386462/
Abstract

The aim of the present study was to produce exo-polygalacturonase from potent soil isolate by submerged fermentation and its application for fruit juice treatment. Pectinase producing strains were selectively isolated from pectin industry waste. A selected isolate C2 was found to produce significant amount of exo-polygalacturonase. The isolate was identified as Paecilomyces variotii on the basis of morphological characteristics and 18S rRNA gene sequence analysis. The exo-polygalacturonase produced by the isolate was purified by ammonium sulphate precipitation, size exclusion chromatography and ion exchange chromatography. The purified enzyme had MW of 39.4 kD based on SDS PAGE. Under partially optimized conditions, purified exo-polygalacturonase showed specific activity of 98.49 U/mg protein at pH 6.0 and 30°C. The enzyme was comparatively stable from 10 to 30°C and the activity decreased with increasing temperature. Purified enzyme brought about considerable reduction in viscosity of fruit juice samples.

摘要

本研究旨在通过深层发酵从有潜力的土壤分离株中生产外聚半乳糖醛酸酶，并将其应用于果汁处理。从果胶工业废物中选择性地分离出产果胶酶的菌株。发现一株分离株 C2 能大量产生外聚半乳糖醛酸酶。根据形态特征和 18S rRNA 基因序列分析，该分离株被鉴定为多形拟青霉。用硫酸铵沉淀、分子筛层析和离子交换层析对分离株产生的外聚半乳糖醛酸酶进行了纯化。根据 SDS-PAGE，纯化酶的 MW 为 39.4 kD。在部分优化条件下，纯化的外聚半乳糖醛酸酶在 pH 6.0 和 30°C 时表现出 98.49 U/mg 蛋白的比活性。该酶在 10 至 30°C 之间相对稳定，活性随温度升高而降低。纯化酶使果汁样品的粘度显著降低。