• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

对一种世界性真菌（曲霉目）的基因组和遗传学见解

Genomic and Genetic Insights Into a Cosmopolitan Fungus, (Eurotiales).

作者信息

Urquhart Andrew S, Mondo Stephen J, Mäkelä Miia R, Hane James K, Wiebenga Ad, He Guifen, Mihaltcheva Sirma, Pangilinan Jasmyn, Lipzen Anna, Barry Kerrie, de Vries Ronald P, Grigoriev Igor V, Idnurm Alexander

机构信息

School of BioSciences, University of Melbourne, Melbourne, VIC, Australia.

U.S. Department of Energy Joint Genome Institute, Walnut Creek, CA, United States.

出版信息

Front Microbiol. 2018 Dec 13;9:3058. doi: 10.3389/fmicb.2018.03058. eCollection 2018.

DOI:10.3389/fmicb.2018.03058
PMID:30619145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6300479/
Abstract

Species in the genus , a member of the fungal order Eurotiales, are ubiquitous in nature and impact a variety of human endeavors. Here, the biology of one common species, , was explored using genomics and functional genetics. Sequencing the genome of two isolates revealed key genome and gene features in this species. A striking feature of the genome was the two-part nature, featuring large stretches of DNA with normal GC content separated by AT-rich regions, a hallmark of many plant-pathogenic fungal genomes. These AT-rich regions appeared to have been mutated by repeat-induced point (RIP) mutations. We developed methods for genetic transformation of , including forward and reverse genetics as well as crossing techniques. Using transformation and crossing, RIP activity was identified, demonstrating for the first time that RIP is an active process within the order Eurotiales. A consequence of RIP is likely reflected by a reduction in numbers of genes within gene families, such as in cell wall degradation, and reflected by growth limitations on on diverse carbon sources. Furthermore, using these transformation tools we characterized a conserved protein containing a domain of unknown function (DUF1212) and discovered it is involved in pigmentation.

摘要

该属的物种是真菌目散囊菌目的成员，在自然界中普遍存在，并影响着人类的多种活动。在此，利用基因组学和功能遗传学对一种常见物种的生物学特性进行了探索。对两个分离株的基因组进行测序，揭示了该物种的关键基因组和基因特征。该基因组的一个显著特征是其两部分的性质，具有大片正常GC含量的DNA区域，被富含AT的区域隔开，这是许多植物致病真菌基因组的一个标志。这些富含AT的区域似乎已被重复诱导点突变（RIP）所突变。我们开发了该物种的遗传转化方法，包括正向和反向遗传学以及杂交技术。通过转化和杂交，确定了RIP活性，首次证明RIP在散囊菌目内是一个活跃的过程。RIP的一个结果可能表现为基因家族中基因数量的减少，如细胞壁降解相关基因，以及在多种碳源上生长受限。此外，利用这些转化工具，我们对一种含有未知功能结构域（DUF1212）的保守蛋白进行了表征，并发现它与色素沉着有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/9857b05e19fe/fmicb-09-03058-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/ab3bb0d1c18d/fmicb-09-03058-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/e18484ff4c4c/fmicb-09-03058-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/9f45904f2272/fmicb-09-03058-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/19eff8073bcd/fmicb-09-03058-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/7250394f929a/fmicb-09-03058-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/f70f64c5d26a/fmicb-09-03058-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/6b6f780a43d2/fmicb-09-03058-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/26b98cd9233b/fmicb-09-03058-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/3b93d2473b28/fmicb-09-03058-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/78be9bb8b290/fmicb-09-03058-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/9857b05e19fe/fmicb-09-03058-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/ab3bb0d1c18d/fmicb-09-03058-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/e18484ff4c4c/fmicb-09-03058-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/9f45904f2272/fmicb-09-03058-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/19eff8073bcd/fmicb-09-03058-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/7250394f929a/fmicb-09-03058-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/f70f64c5d26a/fmicb-09-03058-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/6b6f780a43d2/fmicb-09-03058-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/26b98cd9233b/fmicb-09-03058-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/3b93d2473b28/fmicb-09-03058-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/78be9bb8b290/fmicb-09-03058-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ae62/6300479/9857b05e19fe/fmicb-09-03058-g011.jpg

相似文献

1
Genomic and Genetic Insights Into a Cosmopolitan Fungus, (Eurotiales).对一种世界性真菌（曲霉目）的基因组和遗传学见解
Front Microbiol. 2018 Dec 13;9:3058. doi: 10.3389/fmicb.2018.03058. eCollection 2018.
2
Sexual reproduction as the cause of heat resistance in the food spoilage fungus Byssochlamys spectabilis (anamorph Paecilomyces variotii).有性繁殖作为食品腐败真菌壮观丝葚霉（无性型为拟青霉）耐热性的成因。
Appl Environ Microbiol. 2008 Mar;74(5):1613-9. doi: 10.1128/AEM.01761-07. Epub 2008 Jan 11.
3
Characterization of the complete mitochondrial genome of Paecilomyces variotii and comparative evolutionary mitochondriomics of 36 fungi.蛹草拟青霉的线粒体基因组全序列特征及 36 种真菌的比较线粒体基因组进化分析。
Mol Biol Rep. 2024 Mar 6;51(1):390. doi: 10.1007/s11033-024-09330-x.
4
A Polyphasic Approach including Whole Genome Sequencing Reveals sp. nov. as a Cryptic Sister Species to .一种包括全基因组测序在内的多阶段方法揭示了 新物种，它是 的隐秘姐妹物种。
J Fungi (Basel). 2023 Feb 22;9(3):285. doi: 10.3390/jof9030285.
5
Draft Genome Sequence of the Formaldehyde-Resistant Fungus Byssochlamys spectabilis No. 5 (Anamorph Paecilomyces variotii No. 5) (NBRC109023).抗甲醛真菌壮观被孢霉5号菌株（无性型为拟青霉5号菌株）（NBRC109023）的基因组草图序列
Genome Announc. 2014 Jan 9;2(1):e01162-13. doi: 10.1128/genomeA.01162-13.
6
[The cultural features and parasitic characteristics of fungus genus Paecilomyces species Paecilomyces Variotii Bainier (1907).].[拟青霉属变异拟青霉（1907年，拜尼尔）的文化特征和寄生特性。]
Klin Lab Diagn. 2017;62(5):313-316. doi: 10.18821/0869-2084-2017-62-5-313-316.
7
Characterization of the Highly Efficient Acid-Stable Xylanase and β-Xylosidase System from the Fungus ATHUM 8891 ( ATHUM 8891).来自真菌ATHUM 8891的高效酸稳定木聚糖酶和β-木糖苷酶系统的特性分析（ATHUM 8891）
J Fungi (Basel). 2021 May 29;7(6):430. doi: 10.3390/jof7060430.
8
A large transposable element mediates metal resistance in the fungus Paecilomyces variotii.一个大型可转座元件介导真菌多形被孢霉的金属抗性。
Curr Biol. 2022 Mar 14;32(5):937-950.e5. doi: 10.1016/j.cub.2021.12.048. Epub 2022 Jan 20.
9
Inactivation of stress-resistant ascospores of Eurotiales by industrial sanitizers.通过工业消毒剂使散囊菌目中具有抗逆性的分生孢子失活。
Int J Food Microbiol. 2018 Nov 20;285:27-33. doi: 10.1016/j.ijfoodmicro.2018.06.018. Epub 2018 Jun 30.
10
Molecular and biological characterization of a partitivirus from .从. 中分离的部分病毒的分子和生物学特性
J Gen Virol. 2023 Nov;104(11). doi: 10.1099/jgv.0.001925.

引用本文的文献

1
Gene acquisition by giant transposons primes eukaryotes for rapid evolution via horizontal gene transfer.巨型转座子介导的基因获取通过水平基因转移使真核生物能够快速进化。
Sci Adv. 2024 Dec 6;10(49):eadp8738. doi: 10.1126/sciadv.adp8738.
2
What are the 100 most cited fungal genera?被引用次数最多的100个真菌属有哪些？
Stud Mycol. 2024 Jul;108:1-411. doi: 10.3114/sim.2024.108.01. Epub 2024 Jul 15.
3
Molecular and biological characterization of a partitivirus from .从. 中分离的部分病毒的分子和生物学特性

本文引用的文献

1
Analysis of Repeat Induced Point (RIP) Mutations in Indicates Variability in the RIP Process Between Fungal Species.分析重复诱导点（RIP）突变表明真菌物种间 RIP 过程的可变性。
Genetics. 2019 Jan;211(1):89-104. doi: 10.1534/genetics.118.301712. Epub 2018 Nov 2.
2
Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri.通过对黑曲霉属（Aspergillus section Nigri）的基因组测序来研究种间和种内变异。
Nat Genet. 2018 Dec;50(12):1688-1695. doi: 10.1038/s41588-018-0246-1. Epub 2018 Oct 22.
3
Phylogeny of Paecilomyces, the causal agent of pistachio and some other trees dieback disease in Iran.
J Gen Virol. 2023 Nov;104(11). doi: 10.1099/jgv.0.001925.
4
Isolation of strains and their genome sequencing to analyze the mating system of Ophiocordyceps robertsii.分离菌株并对其基因组进行测序，以分析罗伯茨虫草的交配系统。
PLoS One. 2023 May 2;18(5):e0284978. doi: 10.1371/journal.pone.0284978. eCollection 2023.
5
are active eukaryotic transposable elements mobilized by a new family of tyrosine recombinases.它们是被一类新的酪氨酸重组酶所激活的活跃的真核转座因子。
Proc Natl Acad Sci U S A. 2023 Apr 11;120(15):e2214521120. doi: 10.1073/pnas.2214521120. Epub 2023 Apr 6.
6
A Polyphasic Approach including Whole Genome Sequencing Reveals sp. nov. as a Cryptic Sister Species to .一种包括全基因组测序在内的多阶段方法揭示了 新物种，它是 的隐秘姐妹物种。
J Fungi (Basel). 2023 Feb 22;9(3):285. doi: 10.3390/jof9030285.
7
Transcontinental Dispersal of Nonendemic Fungal Pathogens through Wooden Handicraft Imports.跨大陆传播的非本地真菌病原体通过木制手工艺品进口。
mBio. 2022 Aug 30;13(4):e0107522. doi: 10.1128/mbio.01075-22. Epub 2022 Jun 29.
8
Metabolomic profiling, biological evaluation of , the river Nile-derived fungus using epigenetic and OSMAC approaches.使用表观遗传学和OSMAC方法对源自尼罗河的真菌进行代谢组学分析及生物学评估。
RSC Adv. 2021 Feb 9;11(12):6709-6719. doi: 10.1039/d0ra07578g. eCollection 2021 Feb 4.
9
A large accessory genome and high recombination rates may influence global distribution and broad host range of the fungal plant pathogen Claviceps purpurea.一个大型的附属基因组和高重组率可能会影响真菌植物病原体麦角菌的全球分布和广泛的宿主范围。
PLoS One. 2022 Feb 10;17(2):e0263496. doi: 10.1371/journal.pone.0263496. eCollection 2022.
10
FunOrder: A robust and semi-automated method for the identification of essential biosynthetic genes through computational molecular co-evolution.功能订单：一种通过计算分子共进化来鉴定必需生物合成基因的强大且半自动的方法。
PLoS Comput Biol. 2021 Sep 27;17(9):e1009372. doi: 10.1371/journal.pcbi.1009372. eCollection 2021 Sep.
梨孢拟盘多毛孢属的系统发育，引起伊朗开心果和其他一些树木衰退病的病原体。
PLoS One. 2018 Jul 24;13(7):e0200794. doi: 10.1371/journal.pone.0200794. eCollection 2018.
4
Draft Genome Sequence of the Patulin-Producing Fungus Paecilomyces niveus Strain CO7.产棒曲霉素的雪白拟青霉CO7菌株的基因组序列草图
Genome Announc. 2018 Jun 21;6(25):e00556-18. doi: 10.1128/genomeA.00556-18.
5
Spontaneous and CRISPR/Cas9-induced mutation of the osmosensor histidine kinase of the canola pathogen .油菜病原体渗透感受器组氨酸激酶的自发突变和CRISPR/Cas9诱导的突变
Fungal Biol Biotechnol. 2017 Dec 16;4:12. doi: 10.1186/s40694-017-0043-0. eCollection 2017.
6
BUSCO Applications from Quality Assessments to Gene Prediction and Phylogenomics.BUSCO的应用：从质量评估到基因预测和系统发育基因组学
Mol Biol Evol. 2018 Mar 1;35(3):543-548. doi: 10.1093/molbev/msx319.
7
Sit4-Associated Protein is Required for Pathogenicity of Leptosphaeria maculans on Brassica napus.Sit4相关蛋白是核盘菌对甘蓝型油菜致病性所必需的。
Curr Microbiol. 2017 Dec;74(12):1438-1446. doi: 10.1007/s00284-017-1338-3. Epub 2017 Aug 24.
8
complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters.完整基因组序列、重复序列诱导的点突变以及碳水化合物活性酶基因簇的分区
Biotechnol Biofuels. 2017 Jul 3;10:170. doi: 10.1186/s13068-017-0825-x. eCollection 2017.
9
Global analysis of biosynthetic gene clusters reveals vast potential of secondary metabolite production in Penicillium species.全球生物合成基因簇分析揭示了青霉属物种中次级代谢产物产生的巨大潜力。
Nat Microbiol. 2017 Apr 3;2:17044. doi: 10.1038/nmicrobiol.2017.44.
10
Paecilomyces variotii Fungemia in a Patient with Lymphoma Needing Liver Transplant.一名需要肝移植的淋巴瘤患者发生拟青霉血症
Mycopathologia. 2017 Aug;182(7-8):761-765. doi: 10.1007/s11046-017-0131-y. Epub 2017 Apr 1.